| pubmed-article:8934226 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8934226 | lifeskim:mentions | umls-concept:C0017296 | lld:lifeskim |
| pubmed-article:8934226 | lifeskim:mentions | umls-concept:C0028778 | lld:lifeskim |
| pubmed-article:8934226 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:8934226 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:8934226 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:8934226 | lifeskim:mentions | umls-concept:C0205431 | lld:lifeskim |
| pubmed-article:8934226 | lifeskim:mentions | umls-concept:C0442335 | lld:lifeskim |
| pubmed-article:8934226 | pubmed:issue | 17 | lld:pubmed |
| pubmed-article:8934226 | pubmed:dateCreated | 1997-3-3 | lld:pubmed |
| pubmed-article:8934226 | pubmed:abstractText | Cationic polymers can self-assemble with DNA to form polyelectrolyte complexes capable of gene delivery, although biocompatibility of the complexes is generally limited. Here we have used A-B type cationic-hydrophilic block co-polymers to introduce a protective surface hydrophilic shielding following oriented self-assembly with DNA. Block co-polymers of poly(ethylene glycol)-poly-L-lysine (pEG-pLL) and poly-N-(2-hydroxypropyl)methacrylamide-poly(trimethylammonioethyl methacrylate chloride) (pHPMA-pTMAEM) both show spontaneous formation of complexes with DNA. Surface charge measured by zeta potential is decreased compared with equivalent polycation-DNA complexes in each case. Atomic force microscopy shows that pHPMA-pTMAEM/DNA complexes are discrete spheres similar to those formed between DNA and simple polycations, whereas pEG-pLL/DNA complexes adopt an extended structure. Biological properties depend on the charge ratio of formation. At optimal charge ratio, pEG-pLL/DNA complexes show efficient transfection of 293 cells in vitro, while pHPMA-pTMAEM/DNA complexes are more inert. Both block co-polymer-DNA complexes show only limited cytotoxicity. Careful selection of block co-polymer structure can influence the physicochemical and biological properties of the complexes and should permit design of materials for specific applications, including targeted delivery of genes in vivo. | lld:pubmed |
| pubmed-article:8934226 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8934226 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8934226 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8934226 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8934226 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8934226 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8934226 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8934226 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8934226 | pubmed:issn | 1043-0342 | lld:pubmed |
| pubmed-article:8934226 | pubmed:author | pubmed-author:UlbrichKK | lld:pubmed |
| pubmed-article:8934226 | pubmed:author | pubmed-author:SchachtE HEH | lld:pubmed |
| pubmed-article:8934226 | pubmed:author | pubmed-author:SeymourL WLW | lld:pubmed |
| pubmed-article:8934226 | pubmed:author | pubmed-author:WolfertM AMA | lld:pubmed |
| pubmed-article:8934226 | pubmed:author | pubmed-author:TonchevaVV | lld:pubmed |
| pubmed-article:8934226 | pubmed:author | pubmed-author:NazarovaOO | lld:pubmed |
| pubmed-article:8934226 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8934226 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:8934226 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:8934226 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8934226 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8934226 | pubmed:pagination | 2123-33 | lld:pubmed |
| pubmed-article:8934226 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8934226 | pubmed:meshHeading | pubmed-meshheading:8934226-... | lld:pubmed |
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| pubmed-article:8934226 | pubmed:meshHeading | pubmed-meshheading:8934226-... | lld:pubmed |
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| pubmed-article:8934226 | pubmed:meshHeading | pubmed-meshheading:8934226-... | lld:pubmed |
| pubmed-article:8934226 | pubmed:meshHeading | pubmed-meshheading:8934226-... | lld:pubmed |
| pubmed-article:8934226 | pubmed:meshHeading | pubmed-meshheading:8934226-... | lld:pubmed |
| pubmed-article:8934226 | pubmed:meshHeading | pubmed-meshheading:8934226-... | lld:pubmed |
| pubmed-article:8934226 | pubmed:meshHeading | pubmed-meshheading:8934226-... | lld:pubmed |
| pubmed-article:8934226 | pubmed:meshHeading | pubmed-meshheading:8934226-... | lld:pubmed |
| pubmed-article:8934226 | pubmed:meshHeading | pubmed-meshheading:8934226-... | lld:pubmed |
| pubmed-article:8934226 | pubmed:meshHeading | pubmed-meshheading:8934226-... | lld:pubmed |
| pubmed-article:8934226 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8934226 | pubmed:articleTitle | Characterization of vectors for gene therapy formed by self-assembly of DNA with synthetic block co-polymers. | lld:pubmed |
| pubmed-article:8934226 | pubmed:affiliation | CRC Institute for Cancer Studies, University of Birmingham, UK. | lld:pubmed |
| pubmed-article:8934226 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8934226 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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