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pubmed-article:8921424pubmed:abstractTextIn this study we determined TCR alpha and beta chain nucleotide sequences of HLA-Cw* 1601-restricted cytoxic T lymphocyte (CTL) clones obtained from the peripheral blood lymphocytes (PBL) of a melanoma patient. These clones were previously shown to be involved in the recognition of melanoma-associated antigenic epitopes SAYGEPRKL and AARAVFLAL encoded by gene MAGE-1 and BAGE respectively. All (3/3) anti-MAGE-1 CTL clones displayed TCRBV5 usage and one clonotype was found twice, > 1 year apart, in patient's PBL. Two out of three anti-BAGE CTL clones showed the same TCRAV/AJ and TCRBV/BJ combinations and differed in the alpha chain CDR3 for two residues and in the beta chain CDR3 for a single nucleotide which, however, did not change translation. These results suggest a pattern of TCR conservation in CTL selected for recognition of MAGE-1 or BAGE peptides on the autoiogous melanoma.lld:pubmed
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pubmed-article:8921424pubmed:articleTitleConserved TCR usage by HLA-Cw* 1601-restricted T cell clones recognizing melanoma antigens.lld:pubmed
pubmed-article:8921424pubmed:affiliationDivision of Experimental Oncology D, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milano, Italy.lld:pubmed
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