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pubmed-article:8913804pubmed:abstractTextAs part of a WHO multicenter study on Listeria monocytogenes subtyping methods the random amplification of polymorphic DNA (RAPD)-technique was evaluated. Six participants were asked to use a standard protocol to analyse a set of 80 L. monocytogenes strains. This set contained 22 groups of epidemiologically linked isolates and 11 pairs of duplicate strains. Using three different 10-mer primers the median reproducibility of the RAPD-results obtained by the six participants was 86.5% (range 0-100%). Failure in reproducibility was mainly due to results obtained with one particular primer. The number of epidemiological groups found to be homogeneous varied from 1-22 (median 16). However, for some groups an inhomogeneity was found by the majority of participants. The overall correlation between the results from the different participants ranged from 32 to 85%.lld:pubmed
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pubmed-article:8913804pubmed:authorpubmed-author:RussellE GEGlld:pubmed
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pubmed-article:8913804pubmed:articleTitleThe WHO multicenter study on Listeria monocytogenes subtyping: random amplification of polymorphic DNA (RAPD).lld:pubmed
pubmed-article:8913804pubmed:affiliationMicrobiological Laboratory for Health Protection, National Institute of Public Health and the Environment, Bilthoven, Netherlands. Karel.Wernars@rivm.nllld:pubmed
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