| pubmed-article:8910316 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C0002520 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C0006100 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C1145667 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C2825311 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C2349209 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C0205117 | lld:lifeskim |
| pubmed-article:8910316 | lifeskim:mentions | umls-concept:C0596072 | lld:lifeskim |
| pubmed-article:8910316 | pubmed:issue | 44 | lld:pubmed |
| pubmed-article:8910316 | pubmed:dateCreated | 1996-12-26 | lld:pubmed |
| pubmed-article:8910316 | pubmed:abstractText | To obtain data of the bradykinin B2 receptor's agonist binding site, we used a combined approach of affinity labeling and "immunoidentification" of receptor fragments generated by cyanogen bromide cleavage. Domain-specific antibodies to the various extracellular receptor domains were applied to detect receptor fragments with covalently attached [125I-Tyr8]bradykinin. As a cross-linker we used the homobifunctional reagent disuccinimidyl tartarate (DST), which reacts preferentially with primary amines. With this technique a [125I-Tyr8]bradykinin-labeled receptor fragment derived from the third extracellular domain was identified. The epsilon-amino group of lysine (Lys172) of the human B2 receptor provides the only primary amino group within this receptor fragment. This strongly suggests that DST attached the N-terminal amino group of [Tyr8]bradykinin to Lys172 of the human B2 receptor. Next we asked whether DST attaches [Tyr8]bradykinin to the analogous residue, Lys174 of the rat B2 receptor, which is 81% identical to the human B2 receptor, and we attempted to label the wild-type rat B2 receptor and a rat B2 receptor mutant where Lys174 had been exchanged for alanine. Affinity labeling of the wild-type rat B2 receptor worked efficiently, whereas DST did not attach detectable amounts of [125I-Tyr8]bradykinin to the K174A rat B2 receptor mutant. Taken together these observations indicate that the N-terminal amino group of [Tyr8]bradykinin is bound to analogous positions of the rat and of the human B2 receptor, i.e. [Tyr8]bradykinin's N terminus is bound adjacent to Lys172 of the human and Lys174 of the rat B2 receptor. | lld:pubmed |
| pubmed-article:8910316 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8910316 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910316 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8910316 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910316 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910316 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910316 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910316 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910316 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910316 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910316 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8910316 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8910316 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8910316 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:8910316 | pubmed:author | pubmed-author:Müller-Esterl... | lld:pubmed |
| pubmed-article:8910316 | pubmed:author | pubmed-author:JarnaginKK | lld:pubmed |
| pubmed-article:8910316 | pubmed:author | pubmed-author:QuittererUU | lld:pubmed |
| pubmed-article:8910316 | pubmed:author | pubmed-author:AbdAllaSS | lld:pubmed |
| pubmed-article:8910316 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8910316 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8910316 | pubmed:volume | 271 | lld:pubmed |
| pubmed-article:8910316 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8910316 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8910316 | pubmed:pagination | 27382-7 | lld:pubmed |
| pubmed-article:8910316 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:8910316 | pubmed:meshHeading | pubmed-meshheading:8910316-... | lld:pubmed |
| pubmed-article:8910316 | pubmed:meshHeading | pubmed-meshheading:8910316-... | lld:pubmed |
| pubmed-article:8910316 | pubmed:meshHeading | pubmed-meshheading:8910316-... | lld:pubmed |
| pubmed-article:8910316 | pubmed:meshHeading | pubmed-meshheading:8910316-... | lld:pubmed |
| pubmed-article:8910316 | pubmed:meshHeading | pubmed-meshheading:8910316-... | lld:pubmed |
| pubmed-article:8910316 | pubmed:meshHeading | pubmed-meshheading:8910316-... | lld:pubmed |
| pubmed-article:8910316 | pubmed:meshHeading | pubmed-meshheading:8910316-... | lld:pubmed |
| pubmed-article:8910316 | pubmed:meshHeading | pubmed-meshheading:8910316-... | lld:pubmed |
| pubmed-article:8910316 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8910316 | pubmed:articleTitle | The N-terminal amino group of [Tyr8]bradykinin is bound adjacent to analogous amino acids of the human and rat B2 receptor. | lld:pubmed |
| pubmed-article:8910316 | pubmed:affiliation | Institute of Physiological Chemistry and Pathobiochemistry, University of Mainz, 55099 Germany. | lld:pubmed |
| pubmed-article:8910316 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8910316 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8910316 | lld:pubmed |
