| pubmed-article:8905474 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8905474 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:8905474 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:8905474 | lifeskim:mentions | umls-concept:C0036770 | lld:lifeskim |
| pubmed-article:8905474 | lifeskim:mentions | umls-concept:C2610216 | lld:lifeskim |
| pubmed-article:8905474 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:8905474 | pubmed:dateCreated | 1997-3-11 | lld:pubmed |
| pubmed-article:8905474 | pubmed:abstractText | Depletion of intracellular Ca2+ stores activates an influx of Ca2+ from the extracellular medium. This capacitative Ca2+ entry as originally proposed by Putney in 1986 can be studied with drugs that inhibit sarco/endoplasmic reticulum ATPase. In the present study we examined the effects of depletion of internal Ca2+ stores on Ca2+ influx in rat Sertoli cells utilizing thapsigargin and cyclopiazonic acid. Both inhibitors induced a rapid and dose-dependent rise in [Ca2+]i that was dependent on an influx of Ca2+ from the extracellular medium since it was rapidly blocked by the addition of the Ca2+ chelating agent EGTA. In the absence of external Ca2+ thapsigargin and cyclopiazonic acid still produced an increase in [Ca2+]i that was lower than that observed in Ca2+ medium and was transient since [Ca2+]i returned to basal levels by few minutes. In these experimental conditions readdition of Ca2+ induced a rapid rise in [Ca2+]i supporting a role for Ca2+ influx. Increase of plasma membrane permeability to Ca2+ induced by thapsigargin and cyclopiazonic acid were confirmed by the ability of Mn2+ to permeate through Ca2+ channels and to quench intracellular fura-2 fluorescence after challenge with these inhibitors. Mn2+ induced influx was blocked by La3+, a well known blocker of Ca2+ channels. These results demonstrate that internal Ca2+ stores depletion induce Ca2+ influx from the extracellular medium in rat Sertoli cells providing evidence for the existence of capacitative Ca2+ entry in these cells. | lld:pubmed |
| pubmed-article:8905474 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8905474 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8905474 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8905474 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8905474 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8905474 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8905474 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8905474 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8905474 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8905474 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8905474 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:8905474 | pubmed:issn | 0391-4097 | lld:pubmed |
| pubmed-article:8905474 | pubmed:author | pubmed-author:Di VirgilioFF | lld:pubmed |
| pubmed-article:8905474 | pubmed:author | pubmed-author:ForestaCC | lld:pubmed |
| pubmed-article:8905474 | pubmed:author | pubmed-author:BordonPP | lld:pubmed |
| pubmed-article:8905474 | pubmed:author | pubmed-author:RossatoMM | lld:pubmed |
| pubmed-article:8905474 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8905474 | pubmed:volume | 19 | lld:pubmed |
| pubmed-article:8905474 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8905474 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8905474 | pubmed:pagination | 516-23 | lld:pubmed |
| pubmed-article:8905474 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:meshHeading | pubmed-meshheading:8905474-... | lld:pubmed |
| pubmed-article:8905474 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8905474 | pubmed:articleTitle | Capacitative calcium entry in rat Sertoli cells. | lld:pubmed |
| pubmed-article:8905474 | pubmed:affiliation | III Cattedra di Patologia Medica, University of Padova, Italy. | lld:pubmed |
| pubmed-article:8905474 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8905474 | lld:pubmed |
