| pubmed-article:8889014 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8889014 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:8889014 | lifeskim:mentions | umls-concept:C0002475 | lld:lifeskim |
| pubmed-article:8889014 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
| pubmed-article:8889014 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:8889014 | pubmed:dateCreated | 1997-2-4 | lld:pubmed |
| pubmed-article:8889014 | pubmed:abstractText | Oligodeoxyribonucleotides (ODNs) conjugated with mitomycin C (MC) via (-CH2-)n tethers of different lengths (n = 6, 12) to their terminal 5'-phosphate were synthesized, and their interaction with target complementary single-stranded DNA oligonucleotides was investigated. MC, a clinically used natural anticancer drug, is known to act as a bioreductive alkylating agent of duplex DNA with a remarkable preference for 5'-d(CG) sequences. The usual enzymatic bioreductive techniques known to trigger MC to alkylate DNA were employed in the reaction between the MC-oligonucleotide conjugates and their targets radiolabeled by 32P at their 5'-phosphate. A slow-moving radiolabeled product, detected by polyacrylamide gel electrophoresis using phosphorimaging techniques, was obtained in 15-25% yield with complementary DNA as target. Formation of these products was dependent upon complementary duplex formation. Evidence is presented that the DNA target is alkylated by the mitomycin C moiety of the ODN conjugate at the 2-amino group of a guanine base. These findings suggest that the MC-ODN conjugates may be useful specific inhibitors of cellular or viral gene expression. To our knowledge this is the first report on ODN conjugates of a reductively activated drug of known therapeutic value. | lld:pubmed |
| pubmed-article:8889014 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8889014 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8889014 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8889014 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8889014 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8889014 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8889014 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8889014 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8889014 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8889014 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8889014 | pubmed:issn | 1043-1802 | lld:pubmed |
| pubmed-article:8889014 | pubmed:author | pubmed-author:TomaszMM | lld:pubmed |
| pubmed-article:8889014 | pubmed:author | pubmed-author:MaruendaHH | lld:pubmed |
| pubmed-article:8889014 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8889014 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:8889014 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8889014 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8889014 | pubmed:pagination | 541-4 | lld:pubmed |
| pubmed-article:8889014 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8889014 | pubmed:meshHeading | pubmed-meshheading:8889014-... | lld:pubmed |
| pubmed-article:8889014 | pubmed:meshHeading | pubmed-meshheading:8889014-... | lld:pubmed |
| pubmed-article:8889014 | pubmed:meshHeading | pubmed-meshheading:8889014-... | lld:pubmed |
| pubmed-article:8889014 | pubmed:meshHeading | pubmed-meshheading:8889014-... | lld:pubmed |
| pubmed-article:8889014 | pubmed:meshHeading | pubmed-meshheading:8889014-... | lld:pubmed |
| pubmed-article:8889014 | pubmed:meshHeading | pubmed-meshheading:8889014-... | lld:pubmed |
| pubmed-article:8889014 | pubmed:meshHeading | pubmed-meshheading:8889014-... | lld:pubmed |
| pubmed-article:8889014 | pubmed:meshHeading | pubmed-meshheading:8889014-... | lld:pubmed |
| pubmed-article:8889014 | pubmed:meshHeading | pubmed-meshheading:8889014-... | lld:pubmed |
| pubmed-article:8889014 | pubmed:meshHeading | pubmed-meshheading:8889014-... | lld:pubmed |
| pubmed-article:8889014 | pubmed:articleTitle | Antisense sequence-directed cross-linking of DNA oligonucleotides by mitomycin C. | lld:pubmed |
| pubmed-article:8889014 | pubmed:affiliation | Department of Chemistry, Hunter College, City University of New York, New York 10021, USA. | lld:pubmed |
| pubmed-article:8889014 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8889014 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
