pubmed-article:8876448 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8876448 | lifeskim:mentions | umls-concept:C0007427 | lld:lifeskim |
pubmed-article:8876448 | lifeskim:mentions | umls-concept:C1521747 | lld:lifeskim |
pubmed-article:8876448 | lifeskim:mentions | umls-concept:C0201699 | lld:lifeskim |
pubmed-article:8876448 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
pubmed-article:8876448 | pubmed:issue | 1 | lld:pubmed |
pubmed-article:8876448 | pubmed:dateCreated | 1997-3-5 | lld:pubmed |
pubmed-article:8876448 | pubmed:abstractText | A rapid and simple method for analyzing cathepsin D in breast tissue based on capillary zone electrophoresis (CZE) is described. After incubating the tissue extracts with hemoglobin as a substrate, a specific peptide is cleaved and separated by CZE in less than 5 min. This peptide is not produced by the action of pepsin or trypsin. It is inhibited by the addition of pepstatin, a specific inhibitor for cathepsin D. Human hemoglobin acted as a better substrate than bovine hemoglobin. The test compared well to a radioimmunoassay. We have shown that peptides can be stacked by the use of acetonitrile. The method demonstrates the advantages of CZE for assay of proteolytic enzymes in general. | lld:pubmed |
pubmed-article:8876448 | pubmed:language | eng | lld:pubmed |
pubmed-article:8876448 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8876448 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:8876448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8876448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8876448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8876448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8876448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8876448 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8876448 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8876448 | pubmed:month | Aug | lld:pubmed |
pubmed-article:8876448 | pubmed:issn | 1572-6495 | lld:pubmed |
pubmed-article:8876448 | pubmed:author | pubmed-author:ShihabiZ KZK | lld:pubmed |
pubmed-article:8876448 | pubmed:author | pubmed-author:KuteT ETE | lld:pubmed |
pubmed-article:8876448 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8876448 | pubmed:day | 9 | lld:pubmed |
pubmed-article:8876448 | pubmed:volume | 683 | lld:pubmed |
pubmed-article:8876448 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8876448 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8876448 | pubmed:pagination | 125-31 | lld:pubmed |
pubmed-article:8876448 | pubmed:dateRevised | 2007-10-16 | lld:pubmed |
pubmed-article:8876448 | pubmed:meshHeading | pubmed-meshheading:8876448-... | lld:pubmed |
pubmed-article:8876448 | pubmed:meshHeading | pubmed-meshheading:8876448-... | lld:pubmed |
pubmed-article:8876448 | pubmed:meshHeading | pubmed-meshheading:8876448-... | lld:pubmed |
pubmed-article:8876448 | pubmed:meshHeading | pubmed-meshheading:8876448-... | lld:pubmed |
pubmed-article:8876448 | pubmed:meshHeading | pubmed-meshheading:8876448-... | lld:pubmed |
pubmed-article:8876448 | pubmed:meshHeading | pubmed-meshheading:8876448-... | lld:pubmed |
pubmed-article:8876448 | pubmed:meshHeading | pubmed-meshheading:8876448-... | lld:pubmed |
pubmed-article:8876448 | pubmed:meshHeading | pubmed-meshheading:8876448-... | lld:pubmed |
pubmed-article:8876448 | pubmed:meshHeading | pubmed-meshheading:8876448-... | lld:pubmed |
pubmed-article:8876448 | pubmed:year | 1996 | lld:pubmed |
pubmed-article:8876448 | pubmed:articleTitle | Analysis of cathepsin D from breast tissues by capillary electrophoresis. | lld:pubmed |
pubmed-article:8876448 | pubmed:affiliation | Department of Pathology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA. | lld:pubmed |
pubmed-article:8876448 | pubmed:publicationType | Journal Article | lld:pubmed |