| pubmed-article:8874020 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8874020 | lifeskim:mentions | umls-concept:C1704336 | lld:lifeskim |
| pubmed-article:8874020 | lifeskim:mentions | umls-concept:C0443254 | lld:lifeskim |
| pubmed-article:8874020 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:8874020 | lifeskim:mentions | umls-concept:C0728938 | lld:lifeskim |
| pubmed-article:8874020 | lifeskim:mentions | umls-concept:C0871161 | lld:lifeskim |
| pubmed-article:8874020 | lifeskim:mentions | umls-concept:C1140999 | lld:lifeskim |
| pubmed-article:8874020 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8874020 | pubmed:dateCreated | 1997-3-11 | lld:pubmed |
| pubmed-article:8874020 | pubmed:abstractText | We show prolonged contraction of permeabilized muscle fibers of the frog during which structural order, as judged from low-angle x-ray diffraction, was preserved by means of partial cross-linking of the fibers using the zero-length cross-linker 1-ethyl-3-[3-dimethylamino)propyl]carbodiimide. Ten to twenty percent of the myosin cross-bridges were cross-linked, allowing the remaining 80-90% to cycle and generate force. These fibers displayed a well-preserved sarcomeric order and mechanical characteristics similar to those of intact muscle fibers. The intensity of the brightest meridional reflection at 14.5 nm, resulting from the projection of cross-bridges evenly spaced along the myofilament length, decreased by 60% as a relaxed fiber was deprived of ATP and entered the rigor state. Upon activation of a rigorized fiber by the addition of ATP, the intensity of this reflection returned to 97% of the relaxed value, suggesting that the overall orientation of cross-bridges in the active muscle was more perpendicular to the filament axis than in rigor. Following a small-amplitude length step applied to the active fibers, the reflection intensity decreased for both releases and stretches. In rigor, however, a small stretch increased the amplitude of the reflection by 35%. These findings show the close link between cross-bridge orientation and tension changes. | lld:pubmed |
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| pubmed-article:8874020 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8874020 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8874020 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8874020 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8874020 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:8874020 | pubmed:issn | 0006-3495 | lld:pubmed |
| pubmed-article:8874020 | pubmed:author | pubmed-author:BrownPP | lld:pubmed |
| pubmed-article:8874020 | pubmed:author | pubmed-author:FerencziM AMA | lld:pubmed |
| pubmed-article:8874020 | pubmed:author | pubmed-author:WebbMM | lld:pubmed |
| pubmed-article:8874020 | pubmed:author | pubmed-author:BershitskySS | lld:pubmed |
| pubmed-article:8874020 | pubmed:author | pubmed-author:TsaturyanAA | lld:pubmed |
| pubmed-article:8874020 | pubmed:author | pubmed-author:BershitskayaO... | lld:pubmed |
| pubmed-article:8874020 | pubmed:author | pubmed-author:MashanovGG | lld:pubmed |
| pubmed-article:8874020 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8874020 | pubmed:volume | 71 | lld:pubmed |
| pubmed-article:8874020 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8874020 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8874020 | pubmed:pagination | 1462-74 | lld:pubmed |
| pubmed-article:8874020 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:8874020 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8874020 | pubmed:articleTitle | Mechanical and structural properties underlying contraction of skeletal muscle fibers after partial 1-ethyl-3-[3-dimethylamino)propyl]carbodiimide cross-linking. | lld:pubmed |
| pubmed-article:8874020 | pubmed:affiliation | Institute of Physiology, Russian Academy of Sciences, Yekaterinburg, Russia. | lld:pubmed |
| pubmed-article:8874020 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8874020 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:8874020 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8874020 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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