| pubmed-article:8864654 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8864654 | lifeskim:mentions | umls-concept:C0014257 | lld:lifeskim |
| pubmed-article:8864654 | lifeskim:mentions | umls-concept:C1801960 | lld:lifeskim |
| pubmed-article:8864654 | lifeskim:mentions | umls-concept:C0011847 | lld:lifeskim |
| pubmed-article:8864654 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:8864654 | pubmed:dateCreated | 1997-3-5 | lld:pubmed |
| pubmed-article:8864654 | pubmed:abstractText | Nitric oxide (NO) is believed to mediate the phenomenon known as endothelium-dependent relaxation (EDR). NO synthase produces NO from its precursor, arginine (ARG). We have previously demonstrated impaired EDR in diabetic blood vessels. In this study, we investigated a possible mechanism for defective EDR in experimental diabetes and whether pancreatic islet transplantation could reverse established endothelial dysfunction. Streptozotocin-induced diabetic rats were maintained for 8 or 12 weeks. NO-mediated EDR was assessed in isolated thoracic rings ex vivo. A group of untreated diabetic rats received syngeneic islet transplantation at 8 weeks of diabetes and were tested for EDR after 4 weeks of euglycemia. EDR to acetylcholine was impaired in untreated diabetic rings. Endothelium-independent relaxation to nitroglycerin was unaltered. In vitro incubation of diabetic rings with 3 mM L-ARG (but not D-ARG) improved EDR to acetylcholine in rings from 8-week but not 12-week diabetic rats. L-ARG did not alter EDR in control rings nor relaxation to nitroglycerin in control or diabetic rings. Islet transplantation at 8 weeks of diabetes normalized blood glucose, plasma arginine and total glycosylated hemoglobin while restoring normal EDR. In conclusion, a defect in substrate/supply for NO synthesis is acutely reversed by ARG supplementation at early but not at later stages of diabetes. Also, preemptive surgical intervention with islet transplantation prior to the ARG-insensitive phase is an effective strategy to reverse established endothelial dysfunction in diabetes mellitus. | lld:pubmed |
| pubmed-article:8864654 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8864654 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8864654 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8864654 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8864654 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8864654 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8864654 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8864654 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8864654 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8864654 | pubmed:issn | 0168-8227 | lld:pubmed |
| pubmed-article:8864654 | pubmed:author | pubmed-author:PieperG MGM | lld:pubmed |
| pubmed-article:8864654 | pubmed:author | pubmed-author:JordanMM | lld:pubmed |
| pubmed-article:8864654 | pubmed:author | pubmed-author:AdamsM BMB | lld:pubmed |
| pubmed-article:8864654 | pubmed:author | pubmed-author:RozaA MAM | lld:pubmed |
| pubmed-article:8864654 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8864654 | pubmed:volume | 31 Suppl | lld:pubmed |
| pubmed-article:8864654 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8864654 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8864654 | pubmed:pagination | S157-62 | lld:pubmed |
| pubmed-article:8864654 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:meshHeading | pubmed-meshheading:8864654-... | lld:pubmed |
| pubmed-article:8864654 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8864654 | pubmed:articleTitle | Restoration of vascular endothelial function in diabetes. | lld:pubmed |
| pubmed-article:8864654 | pubmed:affiliation | Department of Transplant Surgery, Medical College of Wisconsin Froedtert Memorial Hospital, Milwaukee 53226, USA. | lld:pubmed |
| pubmed-article:8864654 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8864654 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8864654 | lld:pubmed |
