| pubmed-article:8845566 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8845566 | lifeskim:mentions | umls-concept:C0026820 | lld:lifeskim |
| pubmed-article:8845566 | lifeskim:mentions | umls-concept:C0010738 | lld:lifeskim |
| pubmed-article:8845566 | lifeskim:mentions | umls-concept:C1947951 | lld:lifeskim |
| pubmed-article:8845566 | lifeskim:mentions | umls-concept:C1155937 | lld:lifeskim |
| pubmed-article:8845566 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:8845566 | lifeskim:mentions | umls-concept:C0243111 | lld:lifeskim |
| pubmed-article:8845566 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8845566 | pubmed:dateCreated | 1996-10-18 | lld:pubmed |
| pubmed-article:8845566 | pubmed:abstractText | We investigated the mode of relaxant effects of cytochalasin D, a capping agent of actin filaments, on contractile responses in the rat aorta and chicken gizzard smooth muscles. Cytochalasin D inhibited the contraction induced by high K+ or noradrenaline (10 nM-1 microM) without changing cytosolic Ca2+ level ([Ca2+]i) in the rat aorta. In the absence of external Ca2+, 12-deoxyphorbol 13-isobutylate (DPB) (1 microM) induced sustained contraction without increasing in [Ca2+]i and cytochalasin D also inhibited this contraction. In the permeabilized chicken gizzard smooth muscle, cytochalasin D inhibited the Ca2+ (1-10 microM)-induced contraction. Cytochalasin D also inhibited the Ca(2+)-independent contraction in the muscle which had been thiophosphorylated by ATP gamma S. Cytochalasin D decreased the velocity of superprecipitation in the chicken gizzard native actomyosin (myosin B) affecting neither the level of MLC phosphorylation nor Mg(2+)-ATPase activity. These results suggest that cytochalasin D inhibits smooth muscle contractions without any effect on the Ca(2+)-dependent MLC phosphorylation or subsequent activation of myosin ATPase activity. Based on these evidences, it is concluded that cytochalasin D may inhibit smooth muscle contraction possibly through uncoupling of the force generation from the activated actomyosin Mg(2+)-ATPase. | lld:pubmed |
| pubmed-article:8845566 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8845566 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8845566 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8845566 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8845566 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8845566 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8845566 | pubmed:issn | 0916-8737 | lld:pubmed |
| pubmed-article:8845566 | pubmed:author | pubmed-author:KarakiHH | lld:pubmed |
| pubmed-article:8845566 | pubmed:author | pubmed-author:HoriMM | lld:pubmed |
| pubmed-article:8845566 | pubmed:author | pubmed-author:OzakiHH | lld:pubmed |
| pubmed-article:8845566 | pubmed:author | pubmed-author:SaitoS YSY | lld:pubmed |
| pubmed-article:8845566 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8845566 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:8845566 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8845566 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8845566 | pubmed:pagination | 51-60 | lld:pubmed |
| pubmed-article:8845566 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:8845566 | pubmed:meshHeading | pubmed-meshheading:8845566-... | lld:pubmed |
| pubmed-article:8845566 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8845566 | pubmed:articleTitle | Cytochalasin D inhibits smooth muscle contraction by directly inhibiting contractile apparatus. | lld:pubmed |
| pubmed-article:8845566 | pubmed:affiliation | Department of Veterinary Pharmacology, Graduate School of Agriculture and Life Sciences, University of Tokyo, Japan. | lld:pubmed |
| pubmed-article:8845566 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8845566 | pubmed:publicationType | In Vitro | lld:pubmed |
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