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pubmed-article:8845019pubmed:abstractTextWe have calibrated a solid state RAST assay with affinity purified allergen-specific IgE. We then utilized the calibrated assay to measure the average affinity of individual IgE-containing sera in terms of the average association constant < K > for purified allergen Amb a V. The binding data yielded linear reciprocal plots indicating that the range of affinities of the responding clones was narrow. The range of the average association constant for the IgE-Amb a V complex was 0.9-26 x 10(10) M-1. The average affinity of the corresponding IgG response in the same individual, estimated by inhibition studies of IgE binding, was 10(7) M-1 in one case and lower than 10(6) M-1 in all the other cases.lld:pubmed
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pubmed-article:8845019pubmed:dateRevised2010-11-18lld:pubmed
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pubmed-article:8845019pubmed:articleTitleThe affinity of allergen specific IgE and the competition between IgE and IgG for the allergen in Amb a V sensitive individuals.lld:pubmed
pubmed-article:8845019pubmed:affiliationYonsei University, Seoul, Korea.lld:pubmed
pubmed-article:8845019pubmed:publicationTypeJournal Articlelld:pubmed