| pubmed-article:8819163 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8819163 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
| pubmed-article:8819163 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:8819163 | pubmed:dateCreated | 1997-4-10 | lld:pubmed |
| pubmed-article:8819163 | pubmed:abstractText | Based on observations of solubility and folding properties of peptide 33-mers derived from the beta-sheet domains of platelet factor-4 (PF4), interleukin-8 (IL-8), and growth related protein (Gro-alpha), as well as other beta-sheet-forming peptides, general guidelines have been developed to aid in the design of water soluble, self-association-induced beta-sheet-forming peptides. CD, 1H-NMR, and pulsed field gradient NMR self-diffusion measurements have been used to assess the degree of folding and state of aggregation. PF4 peptide forms native-like beta-sheet tetramers and is sparingly soluble above pH 6. IL-8 peptide is insoluble between pH 4.5 and pH 7.5, yet forms stable, native-like beta-sheet dimers at higher pH. Gro-alpha peptide is soluble at all pH values, yet displays no discernable beta-sheet structure even when diffusion data indicate dimer-tetramer aggregation. A recipe used in the de novo design of water-soluble beta-sheet-forming peptides calls for the peptide to contain 40-50% hydrophobic residues, usually aliphatic ones (I, L, V, A, M) (appropriately paired and mostly but not always alternating with polar residues in the sheet sequence), a positively charged (K, R) to negatively charged (E, D) residue ratio between 4/2 and 6/2, and a noncharged polar residue (N, Q, T, S) composition of about 20% or less. Results on four de novo designed, 33-residue peptides are presented supporting this approach. Under near physiologic conditions, all four peptides are soluble, form beta-sheet structures to varying degrees, and self-associate. One peptide folds as a stable, compact beta-sheet tetramer, whereas the others are transient beta-sheet-containing aggregates. | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8819163 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8819163 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8819163 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8819163 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8819163 | pubmed:issn | 0961-8368 | lld:pubmed |
| pubmed-article:8819163 | pubmed:author | pubmed-author:MayoK HKH | lld:pubmed |
| pubmed-article:8819163 | pubmed:author | pubmed-author:ParkHH | lld:pubmed |
| pubmed-article:8819163 | pubmed:author | pubmed-author:IlyinaEE | lld:pubmed |
| pubmed-article:8819163 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8819163 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:8819163 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8819163 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8819163 | pubmed:pagination | 1301-15 | lld:pubmed |
| pubmed-article:8819163 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:8819163 | pubmed:meshHeading | pubmed-meshheading:8819163-... | lld:pubmed |
| pubmed-article:8819163 | pubmed:meshHeading | pubmed-meshheading:8819163-... | lld:pubmed |
| pubmed-article:8819163 | pubmed:meshHeading | pubmed-meshheading:8819163-... | lld:pubmed |
| pubmed-article:8819163 | pubmed:meshHeading | pubmed-meshheading:8819163-... | lld:pubmed |
| pubmed-article:8819163 | pubmed:meshHeading | pubmed-meshheading:8819163-... | lld:pubmed |
| pubmed-article:8819163 | pubmed:meshHeading | pubmed-meshheading:8819163-... | lld:pubmed |
| pubmed-article:8819163 | pubmed:meshHeading | pubmed-meshheading:8819163-... | lld:pubmed |
| pubmed-article:8819163 | pubmed:meshHeading | pubmed-meshheading:8819163-... | lld:pubmed |
| pubmed-article:8819163 | pubmed:meshHeading | pubmed-meshheading:8819163-... | lld:pubmed |
| pubmed-article:8819163 | pubmed:meshHeading | pubmed-meshheading:8819163-... | lld:pubmed |
| pubmed-article:8819163 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8819163 | pubmed:articleTitle | A recipe for designing water-soluble, beta-sheet-forming peptides. | lld:pubmed |
| pubmed-article:8819163 | pubmed:affiliation | Department of Biochemistry, University of Minnesota, Minneapolis 55455, USA. | lld:pubmed |
| pubmed-article:8819163 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8819163 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8819163 | lld:pubmed |
