| pubmed-article:8798393 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8798393 | lifeskim:mentions | umls-concept:C0034865 | lld:lifeskim |
| pubmed-article:8798393 | lifeskim:mentions | umls-concept:C0033414 | lld:lifeskim |
| pubmed-article:8798393 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:8798393 | lifeskim:mentions | umls-concept:C1704973 | lld:lifeskim |
| pubmed-article:8798393 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:8798393 | lifeskim:mentions | umls-concept:C1705822 | lld:lifeskim |
| pubmed-article:8798393 | lifeskim:mentions | umls-concept:C0348011 | lld:lifeskim |
| pubmed-article:8798393 | lifeskim:mentions | umls-concept:C0121925 | lld:lifeskim |
| pubmed-article:8798393 | pubmed:issue | 37 | lld:pubmed |
| pubmed-article:8798393 | pubmed:dateCreated | 1996-11-7 | lld:pubmed |
| pubmed-article:8798393 | pubmed:abstractText | Genome heterogeneity in retroviruses derives from poor fidelity of the reverse transcriptase (RT) and recombination via RT-catalyzed strand transfer synthesis. RTs lack proofreading ability, and they proficiently extend primers with mismatched termini. Recombination reactions carried out in vitro are accompanied by a high frequency of base substitution errors, suggesting a relationship. Here we provide evidence that misincorporation during RNA-directed DNA synthesis promotes strand transfer recombination. Experiments involved measurement of DNA synthesis, RNase H-directed cleavage, and strand transfer synthesis from preformed mismatched primers on RNA templates by human immunodeficiency virus (HIV) RT in vitro. A significant pause in synthesis occurred from a G(primer). rA(template) mismatch compared to the synthesis from a correctly paired (T.A) primer. The misincorporation-induced pause allowed an unusually large area of RT-RNase H-directed cleavage of the template RNA beneath the primer. Strand transfer to an acceptor molecule with sequence identical to the template RNA was about 50% more efficient than if the primer had had a correctly paired terminus. Overall transfer was measured over a large region of homology. Assuming that enhanced transfer occurs primarily at the site of the mismatch, the actual increase in transfer at that site must have been 1-2 orders of magnitude. Inclusion of a different acceptor molecule with complete complementarity to the originally mismatched 3' primer terminus resulted in an additional 2-fold increase in strand transfer efficiency. Overall, these results suggest the mechanism by which misincorporation during minus strand DNA synthesis in retroviral replication would promote high frequency recombination. | lld:pubmed |
| pubmed-article:8798393 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8798393 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8798393 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8798393 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8798393 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8798393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8798393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8798393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8798393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8798393 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8798393 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8798393 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:8798393 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:8798393 | pubmed:author | pubmed-author:BambaraR ARA | lld:pubmed |
| pubmed-article:8798393 | pubmed:author | pubmed-author:WuWW | lld:pubmed |
| pubmed-article:8798393 | pubmed:author | pubmed-author:FayP JPJ | lld:pubmed |
| pubmed-article:8798393 | pubmed:author | pubmed-author:WisniewskiMM | lld:pubmed |
| pubmed-article:8798393 | pubmed:author | pubmed-author:PalaniappanCC | lld:pubmed |
| pubmed-article:8798393 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8798393 | pubmed:day | 13 | lld:pubmed |
| pubmed-article:8798393 | pubmed:volume | 271 | lld:pubmed |
| pubmed-article:8798393 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8798393 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8798393 | pubmed:pagination | 22331-8 | lld:pubmed |
| pubmed-article:8798393 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:8798393 | pubmed:meshHeading | pubmed-meshheading:8798393-... | lld:pubmed |
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| pubmed-article:8798393 | pubmed:meshHeading | pubmed-meshheading:8798393-... | lld:pubmed |
| pubmed-article:8798393 | pubmed:meshHeading | pubmed-meshheading:8798393-... | lld:pubmed |
| pubmed-article:8798393 | pubmed:meshHeading | pubmed-meshheading:8798393-... | lld:pubmed |
| pubmed-article:8798393 | pubmed:meshHeading | pubmed-meshheading:8798393-... | lld:pubmed |
| pubmed-article:8798393 | pubmed:meshHeading | pubmed-meshheading:8798393-... | lld:pubmed |
| pubmed-article:8798393 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8798393 | pubmed:articleTitle | Misincorporation by HIV-1 reverse transcriptase promotes recombination via strand transfer synthesis. | lld:pubmed |
| pubmed-article:8798393 | pubmed:affiliation | Department of Biochemistry, University of Rochester, Rochester, New York 14642, USA. | lld:pubmed |
| pubmed-article:8798393 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8798393 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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