Linked Life Data

8794772

Source:http://linkedlifedata.com/resource/pubmed/id/8794772

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:8794772rdf:typepubmed:Citationlld:pubmed
pubmed-article:8794772lifeskim:mentionsumls-concept:C0752014lld:lifeskim
pubmed-article:8794772lifeskim:mentionsumls-concept:C0029266lld:lifeskim
pubmed-article:8794772lifeskim:mentionsumls-concept:C1622418lld:lifeskim
pubmed-article:8794772lifeskim:mentionsumls-concept:C0010798lld:lifeskim
pubmed-article:8794772lifeskim:mentionsumls-concept:C1883254lld:lifeskim
pubmed-article:8794772lifeskim:mentionsumls-concept:C0242417lld:lifeskim
pubmed-article:8794772lifeskim:mentionsumls-concept:C0597484lld:lifeskim
pubmed-article:8794772lifeskim:mentionsumls-concept:C0549193lld:lifeskim
pubmed-article:8794772lifeskim:mentionsumls-concept:C0205088lld:lifeskim
pubmed-article:8794772pubmed:issue36lld:pubmed
pubmed-article:8794772pubmed:dateCreated1996-10-23lld:pubmed
pubmed-article:8794772pubmed:abstractTextVitreoscilla cytochrome bo ubiquinol oxidase is similar in some properties to the Escherichia coli enzyme, but unlike the latter, the Vitreoscilla oxidase functions as a primary Na+ pump. When purified Vitreoscilla cytochrome bo is incorporated into liposomes made from Vitreoscilla phospholipids and energized with a quinol substrate, it translocates Na+, not H+, across the vesicle membrane. Since protonophores CCCP (carbonyl cyanide m-chlorophenylhydrazone) and DTHB (3,5-di-tert-butyl-4-hydroxybenzaldehyde) stimulated the Na+ pumping, it is unlikely that it is a secondary effect due to the presence of Na+/H+ antiporter activity in the preparations. The efficiency of the Na+ pumping was 3.93 Na+ pumped per O2 consumed when ascorbate/TMPD was used as the substrate. The cytochrome has a K(m) and Kcat for Na+ of 2.9 mM and 277 s-1, respectively. When ferricytochrome c was entrapped within liposomes prepared from Vitreoscilla phospholipids, it was reduced by Q1H2 (ubiquinol-1) but not by ascorbate/TMPD (N,N,N',N'-tetramethyl-1,4-phenylenediamine). Although Q1H2 was oxidized by cytochrome bo in solution at a rate approximately 14 times that of the latter substrate, the rate of accumulation of Na+ within cytochrome bo vesicles driven by the membrane impermeable ascorbate/TMPD was 1.23 times that of the membrane permeable ubiquinol. These data allowed a calculation that in these synthetic proteoliposomes the cytochrome bo molecules are only 51% directed inward; a value of 61% inward-directed was estimated by measuring the ascorbate/TMPD oxidase activity of the proteoliposomes before and after disrupting them with Triton X-100. A random orientation of the E. coli cytochrome bo oxidase in proteoliposomes has also been reported.lld:pubmed
pubmed-article:8794772pubmed:languageenglld:pubmed
pubmed-article:8794772pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:citationSubsetIMlld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8794772pubmed:statusMEDLINElld:pubmed
pubmed-article:8794772pubmed:monthSeplld:pubmed
pubmed-article:8794772pubmed:issn0006-2960lld:pubmed
pubmed-article:8794772pubmed:authorpubmed-author:WebsterD ADAlld:pubmed
pubmed-article:8794772pubmed:authorpubmed-author:ParkCClld:pubmed
pubmed-article:8794772pubmed:authorpubmed-author:CokicPPlld:pubmed
pubmed-article:8794772pubmed:authorpubmed-author:MoonJ YJYlld:pubmed
pubmed-article:8794772pubmed:issnTypePrintlld:pubmed
pubmed-article:8794772pubmed:day10lld:pubmed
pubmed-article:8794772pubmed:volume35lld:pubmed
pubmed-article:8794772pubmed:ownerNLMlld:pubmed
pubmed-article:8794772pubmed:authorsCompleteYlld:pubmed
pubmed-article:8794772pubmed:pagination11895-900lld:pubmed
pubmed-article:8794772pubmed:dateRevised2007-11-15lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:meshHeadingpubmed-meshheading:8794772-...lld:pubmed
pubmed-article:8794772pubmed:year1996lld:pubmed
pubmed-article:8794772pubmed:articleTitleNa(+)-translocating cytochrome bo terminal oxidase from Vitreoscilla: some parameters of its Na+ pumping and orientation in synthetic vesicles.lld:pubmed
pubmed-article:8794772pubmed:affiliationDepartment of Biological, Chemical, and Physical Sciences, Illinois Institute of Technology, Chicago 60616, USA.lld:pubmed
pubmed-article:8794772pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8794772pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
pubmed-article:8794772pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8794772lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8794772lld:pubmed