| pubmed-article:8794379 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8794379 | lifeskim:mentions | umls-concept:C0014644 | lld:lifeskim |
| pubmed-article:8794379 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:8794379 | lifeskim:mentions | umls-concept:C1199284 | lld:lifeskim |
| pubmed-article:8794379 | lifeskim:mentions | umls-concept:C1514798 | lld:lifeskim |
| pubmed-article:8794379 | lifeskim:mentions | umls-concept:C1704387 | lld:lifeskim |
| pubmed-article:8794379 | lifeskim:mentions | umls-concept:C1522642 | lld:lifeskim |
| pubmed-article:8794379 | lifeskim:mentions | umls-concept:C1204024 | lld:lifeskim |
| pubmed-article:8794379 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:8794379 | pubmed:dateCreated | 1996-11-22 | lld:pubmed |
| pubmed-article:8794379 | pubmed:abstractText | We lack a host cell supporting an efficient lytic replication of Epstein-Barr virus (EBV). Recently, we isolated EBV-negative cell clones from the Akata cell line (referred as Akata- [N. Shimizu, A. Tanabe-Tochikura, Y. Kuroiwa, and K. Takada, J. Virol. 68:6069-6073, 1994). Since the parental Akata line is one of the highest EBV producers, we examined whether Akata- cells had become a good host for EBV propagation. The parental Akata cells have about 20 copies of EBV plasmid per cell. A drug resistance gene was inserted into one of them by homologous recombination. The resultant virus preparation, a mixture of wild-type and recombinant EBV, was used to infect Akata- cells. After incubation in the selective medium, drug-resistant Akata- cell clones were isolated and proved to be infected with recombinant EBV only. By treatment of the cells with antiimmunoglobulin antibodies, a large amount of recombinant EBV (i.e., more than 10 microg/1-liter culture) was produced. In contrast, three other B-lymphoma lines, BJAB, Ramos, and Louckes, were nonpermissive for virus replication. These results indicate that Akata- cells are suitable for propagation of recombinant EBV clonally, which becomes a powerful tool for determining EBV genetics and which makes it possible to use EBV as a vector for gene therapy. | lld:pubmed |
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| pubmed-article:8794379 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8794379 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8794379 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8794379 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8794379 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:8794379 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:8794379 | pubmed:author | pubmed-author:ShimizuNN | lld:pubmed |
| pubmed-article:8794379 | pubmed:author | pubmed-author:TakadaKK | lld:pubmed |
| pubmed-article:8794379 | pubmed:author | pubmed-author:YoshiyamaHH | lld:pubmed |
| pubmed-article:8794379 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8794379 | pubmed:volume | 70 | lld:pubmed |
| pubmed-article:8794379 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8794379 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8794379 | pubmed:pagination | 7260-3 | lld:pubmed |
| pubmed-article:8794379 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:8794379 | pubmed:meshHeading | pubmed-meshheading:8794379-... | lld:pubmed |
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| pubmed-article:8794379 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8794379 | pubmed:articleTitle | Clonal propagation of Epstein-Barr virus (EBV) recombinants in EBV-negative Akata cells. | lld:pubmed |
| pubmed-article:8794379 | pubmed:affiliation | Department of Virology and Parasitology, Yamaguchi University School of Medicine, Japan. | lld:pubmed |
| pubmed-article:8794379 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8794379 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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