| pubmed-article:8787391 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8787391 | lifeskim:mentions | umls-concept:C0599840 | lld:lifeskim |
| pubmed-article:8787391 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:8787391 | lifeskim:mentions | umls-concept:C0037592 | lld:lifeskim |
| pubmed-article:8787391 | lifeskim:mentions | umls-concept:C0043193 | lld:lifeskim |
| pubmed-article:8787391 | lifeskim:mentions | umls-concept:C1880371 | lld:lifeskim |
| pubmed-article:8787391 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:8787391 | pubmed:dateCreated | 1996-9-25 | lld:pubmed |
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| pubmed-article:8787391 | pubmed:abstractText | A culture-independent survey of the soil microbial diversity in a clover-grass pasture in southern Wisconsin was conducted by sequence analysis of a universal clone library of genes coding for small-subunit rRNA (rDNA). A rapid and efficient method for extraction of DNA from soils which resulted in highly purified DNA with minimal shearing was developed. Universal small-subunit-rRNA primers were used to amplify DNA extracted from the pasture soil. The PCR products were cloned into pGEM-T, and either hypervariable or conserved regions were sequenced. The relationships of 124 sequences to those of cultured organisms of known phylogeny were determined. Of the 124 clones sequenced, 98.4% were from the domain Bacteria. Two of the rDNA sequences were derived from eukaryotic organelles. Two of the 124 sequences were of nuclear origin, one being fungal and the other a plant sequence. No sequences of the domain Archaea were found. Within the domain, Bacteria, three kingdoms were highly represented: the Proteobacteria (16.1%), the Cytophaga-Flexibacter-Bacteroides group (21.8%), and the low G+C-content gram-positive group (21.8%). Some kingdoms, such as the Thermotogales, the green nonsulfur group, Fusobacteria, and the Spirochaetes, were absent. A large number of the sequences (39.4%) were distributed among several clades that are not among the major taxa described by Olsen et al. (G.J. Olsen, C.R. Woese, and R. Overbeek, J. Bacteriol., 176:1-6, 1994). From the alignments of the sequence data, distance matrices were calculated to display the enormous microbial diversity found in this soil in two ways, as phylogenetic trees and as multidimensional-scaling plots. | lld:pubmed |
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| pubmed-article:8787391 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8787391 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8787391 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8787391 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8787391 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:8787391 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:8787391 | pubmed:author | pubmed-author:JansenJ LJL | lld:pubmed |
| pubmed-article:8787391 | pubmed:author | pubmed-author:O'SullivanK... | lld:pubmed |
| pubmed-article:8787391 | pubmed:author | pubmed-author:TriplettE WEW | lld:pubmed |
| pubmed-article:8787391 | pubmed:author | pubmed-author:NienhuisJJ | lld:pubmed |
| pubmed-article:8787391 | pubmed:author | pubmed-author:BornemanJJ | lld:pubmed |
| pubmed-article:8787391 | pubmed:author | pubmed-author:RumjanekN GNG | lld:pubmed |
| pubmed-article:8787391 | pubmed:author | pubmed-author:SkrochP WPW | lld:pubmed |
| pubmed-article:8787391 | pubmed:author | pubmed-author:PalusJ AJA | lld:pubmed |
| pubmed-article:8787391 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8787391 | pubmed:volume | 62 | lld:pubmed |
| pubmed-article:8787391 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8787391 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8787391 | pubmed:pagination | 1935-43 | lld:pubmed |
| pubmed-article:8787391 | pubmed:dateRevised | 2010-9-10 | lld:pubmed |
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