Linked Life Data

8785472

Source:http://linkedlifedata.com/resource/pubmed/id/8785472

Statements in which the resource exists.
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pubmed-article:8785472pubmed:abstractText4'-Thio-beta-D-oligoribonucleotides (12 mer and 16 mer) containing a mixed base sequence were synthesized via the phosphoramidite solid support approach. These RNA analogs showed very good nuclease resistance as compared with wild-type RNA. Furthermore, 4'-thio-beta-D-oligoribonucleotides were shown to hybridize with a complementary DNA or RNA strand to form a duplex or with a DNA hairpin to form a triple helix. 4'-Thio-RNA binds more tightly to its complementary RNA strand than to its complementary DNA strand. A 4'-thio-RNA:RNA duplex is as stable as a 2'-O-methyl-RNA:RNA duplex. 4'-Thio-RNA, however, forms a 4'-thio-RNA:DNA:DNA triplex with a stability similar to the corresponding triplex with all wild-type DNA.lld:pubmed
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pubmed-article:8785472pubmed:articleTitle4'-Thio-RNA: synthesis of mixed base 4'-thio-oligoribonucleotides, nuclease resistance, and base pairing properties with complementary single and double strand.lld:pubmed
pubmed-article:8785472pubmed:affiliationLaboratoire de Chimie Bio-Organique, URA 488, CNRS, Université de Montpellier II, France.lld:pubmed
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