| pubmed-article:8783903 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8783903 | lifeskim:mentions | umls-concept:C0014442 | lld:lifeskim |
| pubmed-article:8783903 | lifeskim:mentions | umls-concept:C1562632 | lld:lifeskim |
| pubmed-article:8783903 | lifeskim:mentions | umls-concept:C1749889 | lld:lifeskim |
| pubmed-article:8783903 | lifeskim:mentions | umls-concept:C0034378 | lld:lifeskim |
| pubmed-article:8783903 | lifeskim:mentions | umls-concept:C1514697 | lld:lifeskim |
| pubmed-article:8783903 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8783903 | pubmed:dateCreated | 1996-12-3 | lld:pubmed |
| pubmed-article:8783903 | pubmed:abstractText | We evaluated the use of two radiolabeled lambda DNA/Hpa II substrates to detect 5'-->3', 3'-->5' single and double stranded DNA dependent exonuclease and phosphatase activities found as contaminants in restriction and modifying enzyme preparations. Looking for the meaning of the radioactive assays results in a real cloning experience, we performed a cloning simulation assay using the same conditions established for the radioactive assay (enzyme units and pmols of DNA ends). As a result, we found that for degradation percentages of the radioactive DNA substrate per enzyme unit below 0.5, the false positives in the cloning stimulation assay were less than 5%. This conditions could ensure a good performance of the enzyme preparations for cloning experiments. Finally, we described the use of the radiolabeled [gamma 33P] ATP lambda Hpa II DNA substrate to detect 5'-->3' single stranded DNA dependent exonuclease and phosphatase contaminating activities in some critical steps of the purification process of the restriction enzyme Kpn I. | lld:pubmed |
| pubmed-article:8783903 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8783903 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8783903 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8783903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8783903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8783903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8783903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8783903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8783903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8783903 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8783903 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8783903 | pubmed:issn | 0187-4640 | lld:pubmed |
| pubmed-article:8783903 | pubmed:author | pubmed-author:GonzálezEE | lld:pubmed |
| pubmed-article:8783903 | pubmed:author | pubmed-author:MirandaFF | lld:pubmed |
| pubmed-article:8783903 | pubmed:author | pubmed-author:PérezEE | lld:pubmed |
| pubmed-article:8783903 | pubmed:author | pubmed-author:RuppN TNT | lld:pubmed |
| pubmed-article:8783903 | pubmed:author | pubmed-author:TrujilloL ELE | lld:pubmed |
| pubmed-article:8783903 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8783903 | pubmed:volume | 38 | lld:pubmed |
| pubmed-article:8783903 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8783903 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8783903 | pubmed:pagination | 31-7 | lld:pubmed |
| pubmed-article:8783903 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:meshHeading | pubmed-meshheading:8783903-... | lld:pubmed |
| pubmed-article:8783903 | pubmed:articleTitle | Correspondence between radioactive and functional methods in the quality control of DNA restriction and modifying enzymes. | lld:pubmed |
| pubmed-article:8783903 | pubmed:affiliation | Molecular Biology Department, Center for Genetic Engineering and Biotechnology, (CIGB), Havana City, Cuba. | lld:pubmed |
| pubmed-article:8783903 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8783903 | pubmed:publicationType | Comparative Study | lld:pubmed |
