Linked Life Data

8760073

Source:http://linkedlifedata.com/resource/pubmed/id/8760073

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pubmed-article:8760073pubmed:abstractTextSpecific regulatory mechanisms of aldosterone-stimulated Na+ reabsorption through the apical amiloride-sensitive channel are unknown. In this study, we examined the effects of aldosterone on Na+ channel gamma-subunit mRNA levels in cultured rabbit cortical collecting duct cells. With the use of reverse transcriptase-polymerase chain reaction (RT-PCR) with RNA isolated from aldosterone-treated cells and degenerate primers, a 446-base pair (bp) PCR product was amplified and further characterized by nested PCR and sequencing. The nested PCR yielded a predicted 164-bp product. Sequencing of the 446-bp PCR product revealed 83% nucleotide and 91% amino acid identity to the rat colonic Na+ channel gamma-subunit. The relative abundance of Na+ channel mRNA was determined by quantitative PCR after a 24-h aldosterone treatment. The results demonstrate that Na+ channel gamma-subunit mRNA levels were significantly higher (2.6 +/- 0.42) in aldosterone-treated cultures vs. the controls. This increase, however, is less than the aldosterone-induced increase (3.2 +/- 2.0) in the amiloride-sensitive short-circuit current. These results indicate that Na+ channel gamma-subunit mRNA levels are increased by aldosterone and that this increase is likely to be responsible, at least in part, for the aldosterone-induced Na+ current in the kidney.lld:pubmed
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pubmed-article:8760073pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:8760073pubmed:articleTitleAldosterone regulation of sodium channel gamma-subunit mRNA in cortical collecting duct cells.lld:pubmed
pubmed-article:8760073pubmed:affiliationDepartment of Physiology, Dartmouth Medical School, Lebanon, New Hampshire 03756, USA.lld:pubmed
pubmed-article:8760073pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8760073pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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