Linked Life Data

8756483

Source:http://linkedlifedata.com/resource/pubmed/id/8756483

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:8756483rdf:typepubmed:Citationlld:pubmed
pubmed-article:8756483lifeskim:mentionsumls-concept:C0020202lld:lifeskim
pubmed-article:8756483lifeskim:mentionsumls-concept:C0035668lld:lifeskim
pubmed-article:8756483lifeskim:mentionsumls-concept:C0012893lld:lifeskim
pubmed-article:8756483lifeskim:mentionsumls-concept:C0022702lld:lifeskim
pubmed-article:8756483lifeskim:mentionsumls-concept:C0037633lld:lifeskim
pubmed-article:8756483lifeskim:mentionsumls-concept:C0016315lld:lifeskim
pubmed-article:8756483lifeskim:mentionsumls-concept:C0010101lld:lifeskim
pubmed-article:8756483lifeskim:mentionsumls-concept:C1382100lld:lifeskim
pubmed-article:8756483lifeskim:mentionsumls-concept:C0392762lld:lifeskim
pubmed-article:8756483lifeskim:mentionsumls-concept:C0332283lld:lifeskim
pubmed-article:8756483pubmed:issue31lld:pubmed
pubmed-article:8756483pubmed:dateCreated1996-9-18lld:pubmed
pubmed-article:8756483pubmed:abstractTextBinding kinetics in solution of six N,N,N',N'-tetramethyl-5-carboxyrhodamine-labeled oligodeoxyribonucleotide probes to a 101mer target RNA comprising the primer binding site for HIV-1 reverse transcriptase were characterized using fluorescence correlation spectroscopy (FCS). FCS allows a sensitive, non-radioactive real time observation of hybridization of probes to the RNA target in the buffer of choice without separation of free and bound probe. The binding process could directly be monitored by the change in translational diffusion time of the 17mer to 37mer DNA probe upon specific hybridization with the larger RNA target. The characteristic diffusion time through a laser-illuminated open volume element with 0.5 micron in diameter increased from 0.13-0.2 ms (free) to 0.37-0.50 ms (bound), depending on the probe. Hybridization was approximated by biphasic irreversible second-order reaction kinetics, yielding first-phase association rate constants between 3 x 10(4) and 1.5 x 10(6) M-1 s-1 for the different probes. These varying initial rates reflected the secondary structures of probes and target sites, being consistent with a hypothetical binding pathway starting from loop-loop interactions in a kissing complex, and completion of hybridization requiring an additional interaction involving single-stranded regions of both probe and target. FCS thus permits rapid screening for suitable antisense nucleic acids directed against an important target like HIV-1 RNA with low consumption of probes and target.lld:pubmed
pubmed-article:8756483pubmed:languageenglld:pubmed
pubmed-article:8756483pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8756483pubmed:citationSubsetIMlld:pubmed
pubmed-article:8756483pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8756483pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8756483pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8756483pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8756483pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8756483pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8756483pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8756483pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:8756483pubmed:statusMEDLINElld:pubmed
pubmed-article:8756483pubmed:monthAuglld:pubmed
pubmed-article:8756483pubmed:issn0006-2960lld:pubmed
pubmed-article:8756483pubmed:authorpubmed-author:SchwillePPlld:pubmed
pubmed-article:8756483pubmed:authorpubmed-author:WalkerW EWElld:pubmed
pubmed-article:8756483pubmed:authorpubmed-author:Oehlenschläge...lld:pubmed
pubmed-article:8756483pubmed:issnTypePrintlld:pubmed
pubmed-article:8756483pubmed:day6lld:pubmed
pubmed-article:8756483pubmed:volume35lld:pubmed
pubmed-article:8756483pubmed:ownerNLMlld:pubmed
pubmed-article:8756483pubmed:authorsCompleteYlld:pubmed
pubmed-article:8756483pubmed:pagination10182-93lld:pubmed
pubmed-article:8756483pubmed:dateRevised2006-11-15lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:meshHeadingpubmed-meshheading:8756483-...lld:pubmed
pubmed-article:8756483pubmed:year1996lld:pubmed
pubmed-article:8756483pubmed:articleTitleQuantitative hybridization kinetics of DNA probes to RNA in solution followed by diffusional fluorescence correlation analysis.lld:pubmed
pubmed-article:8756483pubmed:affiliationMax-Planck-Institute for Biophysical Chemistry, Department of Biochemical Kinetics, Göttingen, Germany.lld:pubmed
pubmed-article:8756483pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8756483pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:8756483lld:pubmed