| pubmed-article:8740835 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8740835 | lifeskim:mentions | umls-concept:C0012888 | lld:lifeskim |
| pubmed-article:8740835 | lifeskim:mentions | umls-concept:C1148824 | lld:lifeskim |
| pubmed-article:8740835 | pubmed:issue | 5-6 | lld:pubmed |
| pubmed-article:8740835 | pubmed:dateCreated | 1996-10-21 | lld:pubmed |
| pubmed-article:8740835 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8740835 | pubmed:abstractText | A thermostable DNA polymerase, the Bst DNA polymerase I, from Bacillus stearothermophilus N3468 was prepared to near-homogeneity. The dominant species of the Bst DNA polymerase I preparation sized about 97 kDa when analyzed on SDS polyacrylamide gels. The Bst polA gene that codes for Bst polymerase I was cloned and sequenced. Comparative sequence analysis showed that all three conserved 3'-->5' exonuclease motifs found in E. coli DNA polymerase I were missing in Bst DNA polymerase I. This cast doubt on the existence of a 3'-->5' exonuclease function in that enzyme. Four biochemical assays were used to measure exonuclease activities of Bst DNA polymerase I, testing both full-length Bst polymerase I and the Bst large fragment which lacks the N-terminal 5'-->3' exonuclease domain. These exonuclease assays demonstrated that Bst DNA polymerase I only contained a double-strand dependent 5'-->3' exonuclease activity but lacked any detectable 3'-->5' proofreading exonuclease activity. The lack of 3'-->5' exonuclease function in a variety of thermostable repair DNA polymerases may reflect enhancement of thermostability at the expense of proofreading activity. | lld:pubmed |
| pubmed-article:8740835 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8740835 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8740835 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8740835 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8740835 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8740835 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8740835 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8740835 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8740835 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8740835 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:8740835 | pubmed:author | pubmed-author:WardJ MJM | lld:pubmed |
| pubmed-article:8740835 | pubmed:author | pubmed-author:NOEDLNN | lld:pubmed |
| pubmed-article:8740835 | pubmed:author | pubmed-author:BennerJ SJS | lld:pubmed |
| pubmed-article:8740835 | pubmed:author | pubmed-author:KongHH | lld:pubmed |
| pubmed-article:8740835 | pubmed:author | pubmed-author:AliottaJ MJM | lld:pubmed |
| pubmed-article:8740835 | pubmed:author | pubmed-author:PelletierJ... | lld:pubmed |
| pubmed-article:8740835 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:8740835 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8740835 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8740835 | pubmed:pagination | 185-95 | lld:pubmed |
| pubmed-article:8740835 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:8740835 | pubmed:meshHeading | pubmed-meshheading:8740835-... | lld:pubmed |
| pubmed-article:8740835 | pubmed:meshHeading | pubmed-meshheading:8740835-... | lld:pubmed |
| pubmed-article:8740835 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8740835 | pubmed:articleTitle | Thermostable Bst DNA polymerase I lacks a 3'-->5' proofreading exonuclease activity. | lld:pubmed |
| pubmed-article:8740835 | pubmed:affiliation | New England Biolabs, Inc., Beverly Massachusetts 01915, USA. | lld:pubmed |
| pubmed-article:8740835 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8740835 | pubmed:publicationType | Comparative Study | lld:pubmed |
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