| pubmed-article:8719426 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8719426 | lifeskim:mentions | umls-concept:C0521390 | lld:lifeskim |
| pubmed-article:8719426 | lifeskim:mentions | umls-concept:C0034830 | lld:lifeskim |
| pubmed-article:8719426 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:8719426 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:8719426 | lifeskim:mentions | umls-concept:C0279023 | lld:lifeskim |
| pubmed-article:8719426 | lifeskim:mentions | umls-concept:C0449560 | lld:lifeskim |
| pubmed-article:8719426 | lifeskim:mentions | umls-concept:C2346661 | lld:lifeskim |
| pubmed-article:8719426 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8719426 | pubmed:dateCreated | 1996-10-16 | lld:pubmed |
| pubmed-article:8719426 | pubmed:abstractText | Effects of inorganic lead (Pb2+) on defined subtypes of neuronal nicotinic acetylcholine receptors have been investigated. Voltage clamp experiments have been performed on Xenopus oocytes expressing alpha 3 beta 2, alpha 3 beta 4 and alpha 4 beta 2 neuronal nicotinic acetylcholine receptor subunit combinations. In oocytes expressing the alpha 3 beta 2 subunit combination Pb2+ enhances the peak amplitude of nicotinic acetylcholine receptor-mediated inward currents evoked by superfusion with 100 microM acetylcholine. At concentrations of 1-250 microM, Pb2+ potentiates alpha 3 beta 2 receptor-mediated inward current concentration dependently by a factor of 1.1-11.0. Inward currents evoked by low (3 microM) and high (1 mM) concentrations of acetylcholine are potentiated to a similar extent. Conversely, in oocytes expressing the alpha 3 beta 4 subunit combination Pb2+ inhibits the nicotinic receptor-mediated inward currents evoked with 100 microM acetylcholine. Inhibitory effects are observed in the concentration range of 1 nM-100 microM Pb2+ but the degree of inhibition varies between oocytes. A similar inhibition of the alpha 4 beta 2 nicotinic receptor-mediated inward current by Pb2+ indicates that alpha as well as beta subunits are involved in the potentiating and inhibitory effects. Possible reasons for the variation in the inhibitory effects of Pb2+ on alpha 3 beta 4 and alpha 4 beta 2 nicotinic receptor-mediated inward currents have been investigated and are discussed. The divalent cations Ca2+ and Mg2+ potentiate both alpha 3 beta 2 and alpha 3 beta 4 nicotinic receptor-mediated inward currents. The distinct modulation of receptor function by Pb2+ and by Ca2+ and Mg2+ and the dependence of the modulatory effect of Pb2+ on subunit composition suggest that Pb2+ interacts with multiple sites on the alpha and beta subunits of neuronal nicotinic acetylcholine receptors. | lld:pubmed |
| pubmed-article:8719426 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8719426 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8719426 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8719426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8719426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8719426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8719426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8719426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8719426 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8719426 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8719426 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8719426 | pubmed:issn | 0014-2999 | lld:pubmed |
| pubmed-article:8719426 | pubmed:author | pubmed-author:VijverbergH... | lld:pubmed |
| pubmed-article:8719426 | pubmed:author | pubmed-author:ZwartRR | lld:pubmed |
| pubmed-article:8719426 | pubmed:author | pubmed-author:OortgiesenMM | lld:pubmed |
| pubmed-article:8719426 | pubmed:author | pubmed-author:Van KleefR... | lld:pubmed |
| pubmed-article:8719426 | pubmed:author | pubmed-author:MilikanJ MJM | lld:pubmed |
| pubmed-article:8719426 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8719426 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:8719426 | pubmed:volume | 291 | lld:pubmed |
| pubmed-article:8719426 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8719426 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8719426 | pubmed:pagination | 399-406 | lld:pubmed |
| pubmed-article:8719426 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:meshHeading | pubmed-meshheading:8719426-... | lld:pubmed |
| pubmed-article:8719426 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:8719426 | pubmed:articleTitle | Potentiation and inhibition of subtypes of neuronal nicotinic acetylcholine receptors by Pb2+. | lld:pubmed |
| pubmed-article:8719426 | pubmed:affiliation | Research Institute of Toxicology, Utrecht University, Netherlands. | lld:pubmed |
| pubmed-article:8719426 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8719426 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8719426 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:8719426 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
