| pubmed-article:8705996 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8705996 | lifeskim:mentions | umls-concept:C1705280 | lld:lifeskim |
| pubmed-article:8705996 | lifeskim:mentions | umls-concept:C0007137 | lld:lifeskim |
| pubmed-article:8705996 | lifeskim:mentions | umls-concept:C0460004 | lld:lifeskim |
| pubmed-article:8705996 | lifeskim:mentions | umls-concept:C0525037 | lld:lifeskim |
| pubmed-article:8705996 | lifeskim:mentions | umls-concept:C0376249 | lld:lifeskim |
| pubmed-article:8705996 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:8705996 | pubmed:dateCreated | 1996-9-10 | lld:pubmed |
| pubmed-article:8705996 | pubmed:abstractText | The tumor suppressor gene p16 (CDKN2/MTS-1/INK4A) can be inactivated by multiple genetic mechanisms. We analyzed 29 invasive primary head and neck squamous cell carcinomas (HNSCC) for p16 inactivation with immunohistochemistry utilizing a new monoclonal antibody (mAb), DCS-50. p16 staining of the primary lesions was correlated with genetic analysis including: (a) detailed microsatellite analysis of markers at the p16 locus to detect homozygous deletion; (b) sequence analysis of p16; and (c) Southern blot analysis to determine the methylation status of the 5' CpG island of p16. Twenty-four of 29 (83%) head and neck squamous cell carcinoma tumors displayed an absence of p16 nuclear staining using immunohistochemistry. Of these 24 tumors, we found that 16 (67%) harbored homozygous deletions, 5 (21%) were methylated, 1 displayed a rearrangement at the p16 locus, and 1 displayed a frameshift mutation in exon 1. These data suggest that: (a) inactivation of the p16 tumor suppressor gene is a frequent event in squamous cell carcinomas of the head and neck; (b) p16 is inactivated by several distinct and exclusive events including homozygous deletion, point mutation, and promoter methylation; and (c) immunohistochemical analysis for expression of the p16 gene product is an accurate and relatively simple method for evaluating p16 gene inactivation. | lld:pubmed |
| pubmed-article:8705996 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8705996 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8705996 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8705996 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8705996 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8705996 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8705996 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:BartekJJ | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:KochWW | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:JonesR MRM | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:NawrotBB | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:EbeMM | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:SidranskyDD | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:ReedA LAL | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:SewellDD | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:CairnsPP | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:CalifanoJJ | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:AhrendtSS | lld:pubmed |
| pubmed-article:8705996 | pubmed:author | pubmed-author:WestraW HWH | lld:pubmed |
| pubmed-article:8705996 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8705996 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8705996 | pubmed:volume | 56 | lld:pubmed |
| pubmed-article:8705996 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8705996 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8705996 | pubmed:pagination | 3630-3 | lld:pubmed |
| pubmed-article:8705996 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:8705996 | pubmed:meshHeading | pubmed-meshheading:8705996-... | lld:pubmed |
| pubmed-article:8705996 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8705996 | pubmed:articleTitle | High frequency of p16 (CDKN2/MTS-1/INK4A) inactivation in head and neck squamous cell carcinoma. | lld:pubmed |
| pubmed-article:8705996 | pubmed:affiliation | Department of Otolaryngology Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2196, USA. | lld:pubmed |
| pubmed-article:8705996 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8705996 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8705996 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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