| pubmed-article:8702620 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8702620 | lifeskim:mentions | umls-concept:C0031760 | lld:lifeskim |
| pubmed-article:8702620 | lifeskim:mentions | umls-concept:C0018358 | lld:lifeskim |
| pubmed-article:8702620 | lifeskim:mentions | umls-concept:C1705165 | lld:lifeskim |
| pubmed-article:8702620 | lifeskim:mentions | umls-concept:C1413246 | lld:lifeskim |
| pubmed-article:8702620 | lifeskim:mentions | umls-concept:C1826471 | lld:lifeskim |
| pubmed-article:8702620 | lifeskim:mentions | umls-concept:C1837441 | lld:lifeskim |
| pubmed-article:8702620 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:8702620 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:8702620 | lifeskim:mentions | umls-concept:C2700061 | lld:lifeskim |
| pubmed-article:8702620 | lifeskim:mentions | umls-concept:C1292733 | lld:lifeskim |
| pubmed-article:8702620 | pubmed:issue | 32 | lld:pubmed |
| pubmed-article:8702620 | pubmed:dateCreated | 1996-9-16 | lld:pubmed |
| pubmed-article:8702620 | pubmed:abstractText | Guanylyl cyclase activator proteins GCAP-1 and GCAP-2 (Dizhoor et al. , 1995, Gorczyca et al., 1995) are members of a recently identified subclass of EF-hand type Ca2+-binding proteins that respond to Ca2+ differently than any other known members of the EF-hand superfamily. GCAPs acquire an activating conformation only in their Ca2+-free form. Free Ca2+ concentrations corresponding to levels in dark-adapted vertebrate photoreceptors inhibit the ability of GCAPs to activate photoreceptor guanylyl cyclases (RetGCs). We studied the effects of mutations that block binding of Ca2+ to the EF-hands of GCAP-2. Unlike other EF-hand proteins, which fail to activate their target when their EF-hands are inactivated by mutations, GCAP-2 with any single EF-hand inactivated remains active and is 3-6 times less sensitive to the inhibitory effect of Ca2+. Inactivation of any two or all three EF-hands produces active forms of GCAP-2 that are insensitive to inhibition by physiological intracellular concentrations of Ca2+. Unexpectedly we also found that activation of RetGCs by a Ca2+-insensitive mutant is inhibited by Ca2+-loaded wild type GCAP-2. We propose the following. 1) GCAP-2 can exist in two extreme functional forms: an apo form that activates RetGCs and a Ca2+-loaded form that blocks activation of RetGCs. 2) All three EF-hands of GCAP-2 contribute to the inhibitory effect of Ca2+. 3) Inactivation of two or three EF-hands is sufficient to shift the "activator-inhibitor" transition outside the physiological range of intracellular free Ca2+. | lld:pubmed |
| pubmed-article:8702620 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8702620 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8702620 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8702620 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8702620 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8702620 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8702620 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8702620 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8702620 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:8702620 | pubmed:author | pubmed-author:HurleyJ BJB | lld:pubmed |
| pubmed-article:8702620 | pubmed:author | pubmed-author:DizhoorA MAM | lld:pubmed |
| pubmed-article:8702620 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8702620 | pubmed:day | 9 | lld:pubmed |
| pubmed-article:8702620 | pubmed:volume | 271 | lld:pubmed |
| pubmed-article:8702620 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8702620 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8702620 | pubmed:pagination | 19346-50 | lld:pubmed |
| pubmed-article:8702620 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:8702620 | pubmed:meshHeading | pubmed-meshheading:8702620-... | lld:pubmed |
| pubmed-article:8702620 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8702620 | pubmed:articleTitle | Inactivation of EF-hands makes GCAP-2 (p24) a constitutive activator of photoreceptor guanylyl cyclase by preventing a Ca2+-induced "activator-to-inhibitor" transition. | lld:pubmed |
| pubmed-article:8702620 | pubmed:affiliation | Department of Biochemistry and Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, Washington 98195-7370, USA. | lld:pubmed |
| pubmed-article:8702620 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8702620 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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