| pubmed-article:8668541 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8668541 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:8668541 | lifeskim:mentions | umls-concept:C0796351 | lld:lifeskim |
| pubmed-article:8668541 | lifeskim:mentions | umls-concept:C0039808 | lld:lifeskim |
| pubmed-article:8668541 | lifeskim:mentions | umls-concept:C0016568 | lld:lifeskim |
| pubmed-article:8668541 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:8668541 | pubmed:dateCreated | 1996-8-5 | lld:pubmed |
| pubmed-article:8668541 | pubmed:abstractText | Formamide lowers melting temperatures (Tm) of DNAs linearly by 2.4-2.9 degrees C/mole of formamide (C(F)) depending on the (G+C) composition, helix conformation and state of hydration. The inherent cooperativity of melting is unaffected by the denaturant. dTm/dC(F)for 11 plasmid domains of 0.23 < (G+C)<0.71 generally fit to a linear dependence on (G+C)-content, which, however, is consistent with a (G+C)-independent alteration in the apparent equilibrium constant for thermally induced helix <--> coil transitions. Results indicate that formamide has a destabilizing effect on the helical state, and that sequence-dependent variations in hydration patterns are primarily responsible for small variations in sensitivity to the denaturant. The average unit transition enthalpy delta H(m)[see text for complete expression], exhibits a biphasic dependence on formamide concentration. The initial drop of -0.8 kcal/mol bp at low formamide concentrations is attributable to a delta delta H(m)[see text for complete expression], for exchange of solvent in the vicinity of the helix: displacement by formamide of weakly bound hydrate or counterion. The phenomenological effects are equivalent to lowering the bulk counterion concentration. Poly(dA.dT) exhibits a much lower sensitivity to formamide, due to the specific pattern of tightly bound, immobilized water bridges that buttress the helix from within the narrow minor groove. Tracts of three (A.T)-pairs behave normally, but tracts of six exhibit the same level of reduced sensitivity as the polymer, suggesting a conformational shift as tracts are elongated beyond some critical length [McCarthy J.G. and Rich,A. (1991) Nucleic Acids Res. 19, 3421-3429]. | lld:pubmed |
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| pubmed-article:8668541 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:8668541 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8668541 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8668541 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:8668541 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:8668541 | pubmed:author | pubmed-author:BlakeR DRD | lld:pubmed |
| pubmed-article:8668541 | pubmed:author | pubmed-author:DelcourtS GSG | lld:pubmed |
| pubmed-article:8668541 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8668541 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8668541 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:8668541 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8668541 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8668541 | pubmed:pagination | 2095-103 | lld:pubmed |
| pubmed-article:8668541 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8668541 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8668541 | pubmed:articleTitle | Thermodynamic effects of formamide on DNA stability. | lld:pubmed |
| pubmed-article:8668541 | pubmed:affiliation | Department of Biochemistry, Microbiology and Molecular Biology, University of Maine, Orono, ME 04469-5735, USA. | lld:pubmed |
| pubmed-article:8668541 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8668541 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8668541 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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