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pubmed-article:8652743pubmed:abstractTextDirect comparison of bromodeoxyuridine (BrdUrd) and Ki-67 labelling indices was achieved by selecting similar areas from serial sections of human tumours. Fifteen patients were selected who had been administered BrdUrd in vivo and both proliferation markers were assessed by immunohistochemistry. The data show a good correlation between both BrdUrd LI and MIB-1 LI and Tpot (calculated using the flow cytometry derived duration of S phase) and MIB-1 LI. The contribution of BrdUrd LI to growth fraction varied as a function of proliferation characteristics. In tumours with a high LI, the number of DNA synthesizing cells represented half the growth fraction, whilst in tumours with lower LI's ( < 10%) the ratio of DNA precursor labelled cells as a function of growth fraction fell to between 10% and 20%. Tpot showed a linear correlation with MIB-1/BrdUrd ratio with a slope approaching unity. It was apparent that both intra- and interpatient variation in proliferation index was greater for BrdUrd labelling than for MIB-1 expression.lld:pubmed
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pubmed-article:8652743pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:8652743pubmed:articleTitleDirect comparison of bromodeoxyuridine and Ki-67 labelling indices in human tumours.lld:pubmed
pubmed-article:8652743pubmed:affiliationGray Laboratory Cancer Research Trust, Hampshire, UK.lld:pubmed
pubmed-article:8652743pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8652743pubmed:publicationTypeComparative Studylld:pubmed
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