| pubmed-article:8631499 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8631499 | lifeskim:mentions | umls-concept:C0007603 | lld:lifeskim |
| pubmed-article:8631499 | lifeskim:mentions | umls-concept:C0037868 | lld:lifeskim |
| pubmed-article:8631499 | lifeskim:mentions | umls-concept:C0001455 | lld:lifeskim |
| pubmed-article:8631499 | lifeskim:mentions | umls-concept:C0036488 | lld:lifeskim |
| pubmed-article:8631499 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:8631499 | lifeskim:mentions | umls-concept:C0439799 | lld:lifeskim |
| pubmed-article:8631499 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8631499 | pubmed:dateCreated | 1996-7-3 | lld:pubmed |
| pubmed-article:8631499 | pubmed:abstractText | Ion channels are deeply involved in sperm physiology. In sea urchin sperm cyclic nucleotide levels increase during quimotaxis and in the acrosome reaction (AR). Although cyclic nucleotides are second messengers known to directly or indirectly modulate ion channels, it is not clear how they modulate sperm responses to the egg outer layer. Here, we describe a cAMP regulated K+-selective channel from sea urchin sperm plasma membranes fused into planar bilayers that may have a role during sea urchin sperm quimotaxis and/or the AR. Its single channel conductance in 100 mM KCl is 103 pS. In bi-ionic experiments, the channel displayed a K+/Na+ permeability ratio (PK+/PNa+) of approximately 5. Thus, in sea water its reversal potential would be approximately -13 mV and channel opening would depolarize spermatozoa. The channel has low open probability (Po = 0.8 +/- 0.2% at 0 mV applied voltage) and weak voltage dependence. Channel activity is reversibly up-regulated by cAMP in the cis bilayer side, but not by cGMP. This modulation followed a single Langmuir isotherm with an apparent kd of 200 microM. At this concentration the channel open probability at 0 mV increased up to 11- fold. TEA+ blocked the channel only from the trans side. Also Ba2+ in trans blocked the channel in a voltage-dependent manner. | lld:pubmed |
| pubmed-article:8631499 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8631499 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8631499 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8631499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8631499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8631499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8631499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8631499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8631499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8631499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8631499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8631499 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8631499 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:8631499 | pubmed:issn | 0012-1606 | lld:pubmed |
| pubmed-article:8631499 | pubmed:author | pubmed-author:DarszonAA | lld:pubmed |
| pubmed-article:8631499 | pubmed:author | pubmed-author:LabarcaPP | lld:pubmed |
| pubmed-article:8631499 | pubmed:author | pubmed-author:MoralesEE | lld:pubmed |
| pubmed-article:8631499 | pubmed:author | pubmed-author:ZapataOO | lld:pubmed |
| pubmed-article:8631499 | pubmed:author | pubmed-author:SantoMM | lld:pubmed |
| pubmed-article:8631499 | pubmed:author | pubmed-author:Beltr'anCC | lld:pubmed |
| pubmed-article:8631499 | pubmed:author | pubmed-author:Li'evanoAA | lld:pubmed |
| pubmed-article:8631499 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8631499 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8631499 | pubmed:volume | 174 | lld:pubmed |
| pubmed-article:8631499 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8631499 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8631499 | pubmed:pagination | 271-80 | lld:pubmed |
| pubmed-article:8631499 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
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| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:meshHeading | pubmed-meshheading:8631499-... | lld:pubmed |
| pubmed-article:8631499 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8631499 | pubmed:articleTitle | A cAMP regulated K+-selective channel from the sea urchin sperm plasma membrane. | lld:pubmed |
| pubmed-article:8631499 | pubmed:affiliation | Centro de Estudios Cient'ificos de Santiago, Universidad de Chile, Santiago, Chile. | lld:pubmed |
| pubmed-article:8631499 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8631499 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8631499 | lld:pubmed |
