| pubmed-article:8627761 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8627761 | lifeskim:mentions | umls-concept:C0004120 | lld:lifeskim |
| pubmed-article:8627761 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8627761 | lifeskim:mentions | umls-concept:C0282572 | lld:lifeskim |
| pubmed-article:8627761 | lifeskim:mentions | umls-concept:C0205374 | lld:lifeskim |
| pubmed-article:8627761 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:8627761 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:8627761 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:8627761 | pubmed:dateCreated | 1996-6-21 | lld:pubmed |
| pubmed-article:8627761 | pubmed:abstractText | Different regions of the human astrovirus frameshift signal were cloned into the rhesus rotavirus VP4 gene and evaluated in an infection-transfection transient expression cell culture system. BHK-21 cells, infected with a vaccinia virus that expresses T7 RNA polymerase (vTF7-3), were transfected with the various astrovirus-VP4 constructs. All constructs were driven by a T7 promoter and contained an internal ribosome entry site. Frameshifted and nonframeshifted protein products were immunoprecipitated with VP4 amino- and carboxy-terminal-specific monoclonal antibodies, and their ratios were determined by PhosphorImager analysis. The efficiency of frameshifting was 25 to 28%, significantly greater than the 5 to 7% efficiency reported previously in a cell-free translation system. Coupling of transcription and translation in a cell-free system yielded frameshifting efficiencies threefold greater than that of the uncoupled in vitro system. The presence of the shifty heptamer was an absolute requirement for frameshifting in both cell-free and intact-cell systems, while deletion of the potential downstream pseudoknot region did not affect the efficiency of frameshifting. | lld:pubmed |
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| pubmed-article:8627761 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:8627761 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8627761 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8627761 | pubmed:month | May | lld:pubmed |
| pubmed-article:8627761 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:8627761 | pubmed:author | pubmed-author:LewisT LTL | lld:pubmed |
| pubmed-article:8627761 | pubmed:author | pubmed-author:MatsuiS MSM | lld:pubmed |
| pubmed-article:8627761 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8627761 | pubmed:volume | 70 | lld:pubmed |
| pubmed-article:8627761 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8627761 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8627761 | pubmed:pagination | 2869-75 | lld:pubmed |
| pubmed-article:8627761 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8627761 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8627761 | pubmed:articleTitle | Astrovirus ribosomal frameshifting in an infection-transfection transient expression system. | lld:pubmed |
