| pubmed-article:8626749 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8626749 | lifeskim:mentions | umls-concept:C0025519 | lld:lifeskim |
| pubmed-article:8626749 | lifeskim:mentions | umls-concept:C0431085 | lld:lifeskim |
| pubmed-article:8626749 | lifeskim:mentions | umls-concept:C0000610 | lld:lifeskim |
| pubmed-article:8626749 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:8626749 | lifeskim:mentions | umls-concept:C1261322 | lld:lifeskim |
| pubmed-article:8626749 | lifeskim:mentions | umls-concept:C0024485 | lld:lifeskim |
| pubmed-article:8626749 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:8626749 | lifeskim:mentions | umls-concept:C1553628 | lld:lifeskim |
| pubmed-article:8626749 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:8626749 | pubmed:dateCreated | 1996-6-21 | lld:pubmed |
| pubmed-article:8626749 | pubmed:abstractText | The effect of 6-aminonicotinamide on the metabolism of RIF-1 tumor cells was investigated using 13C and 31P NMR spectroscopy. 6-Aminonicotinamide can be metabolized to 6-amino-NAD(P), a competitive inhibitor of NAD(P)-requiring processes. 40 microM 6-aminonicotinamide led to an inhibition of 6-phosphogluconate dehydrogenase and an accumulation of 6-phosphogluconate. A subsequent accumulation of the 6-phosphogluconate precursor 6-phosphoglucono-delta-lactone was observed in the 13C NMR spectrum. These metabolites were shown to be intracellular, although a small amount of leakage of 6-phosphoglucono-delta-lactone occurred. The intracellular concentrations of 6-phosphogluconate and 6-phosphoglucono-delta-lactone were 1.9 +/- 0.8 micromol/108 cells (+/-1 standard deviation) and 0.8 +/- 0.4 micromol/10(8) cells, respectively, after 15 h. Glucose utilization and lactate production were significantly inhibited by 6-aminonicotinamide (both p < 0.05), indicating inhibition of glycolysis. 31P NMR data showed that phosphocreatine was significantly depleted in cells exposed to 6-aminonicotinamide (p < 0.05). Exposure of RIF-1 cells to 6-aminonicotinamide prior to 3- or 6-Gy x-irradiation induced a supra-additive cell kill, indicating that 6-aminonicotinamide is acting as a radiosensitizer. There was no effect of 6-aminonicotinamide alone or when the drug was given postradiation, suggesting that its mechanism of action may be by inhibition of radiation-induced repair. | lld:pubmed |
| pubmed-article:8626749 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8626749 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8626749 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8626749 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8626749 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8626749 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8626749 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8626749 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8626749 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8626749 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8626749 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:8626749 | pubmed:author | pubmed-author:StreetJ CJC | lld:pubmed |
| pubmed-article:8626749 | pubmed:author | pubmed-author:AlfieriA AAA | lld:pubmed |
| pubmed-article:8626749 | pubmed:author | pubmed-author:KoutcherJ AJA | lld:pubmed |
| pubmed-article:8626749 | pubmed:author | pubmed-author:MahmoodUU | lld:pubmed |
| pubmed-article:8626749 | pubmed:author | pubmed-author:BallonDD | lld:pubmed |
| pubmed-article:8626749 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8626749 | pubmed:day | 23 | lld:pubmed |
| pubmed-article:8626749 | pubmed:volume | 271 | lld:pubmed |
| pubmed-article:8626749 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8626749 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8626749 | pubmed:pagination | 4113-9 | lld:pubmed |
| pubmed-article:8626749 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8626749 | pubmed:meshHeading | pubmed-meshheading:8626749-... | lld:pubmed |
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| pubmed-article:8626749 | pubmed:meshHeading | pubmed-meshheading:8626749-... | lld:pubmed |
| pubmed-article:8626749 | pubmed:meshHeading | pubmed-meshheading:8626749-... | lld:pubmed |
| pubmed-article:8626749 | pubmed:meshHeading | pubmed-meshheading:8626749-... | lld:pubmed |
| pubmed-article:8626749 | pubmed:meshHeading | pubmed-meshheading:8626749-... | lld:pubmed |
| pubmed-article:8626749 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8626749 | pubmed:articleTitle | 13C and 31P NMR investigation of effect of 6-aminonicotinamide on metabolism of RIF-1 tumor cells in vitro. | lld:pubmed |
| pubmed-article:8626749 | pubmed:affiliation | Department of Medical Physics, Sloan-Kettering Cancer Center, New York, New York 10021, USA. | lld:pubmed |
| pubmed-article:8626749 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8626749 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8626749 | lld:pubmed |
