| pubmed-article:8609052 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C0225336 | lld:lifeskim |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C0221920 | lld:lifeskim |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C0032144 | lld:lifeskim |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C0332208 | lld:lifeskim |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C0026559 | lld:lifeskim |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C0018284 | lld:lifeskim |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C1999216 | lld:lifeskim |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:8609052 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:8609052 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8609052 | pubmed:dateCreated | 1996-5-29 | lld:pubmed |
| pubmed-article:8609052 | pubmed:abstractText | Epidermal growth factor (EGF) or transforming growth factor-alpha (TGF-alpha) stimulates cell migration, proliferation and the formation of tube-like structures of human microvascular endothelial cells in culture. Heparin-binding EGF-like growth factor(HB-EGF), which shows 35% homology with EGF/TGF-alpha, is a member of the EGF family, and it is ubiquitous in many tissues and organs. We examined whether or not HB-EGF induced angiogenic responses in human microvascular endothelial cells. HB-EGF inhibited the binding of (125) I-EGF to the EGF receptor and induced autophosphorylation of the receptor on endothelial cells. Exogenous HB-EGF induced the loss of more than 70% of the EGF receptor from the cell surface within 30 min, with similar kinetics to that of EGF. The level of c-fos mRNA markedly increased at 30 min in response to HB-EGF as well as EGF. A gel shift assay demonstrated the activation of the transcription factor p91 by HB-EGF and EGF. This factor directly interacts with EGF receptor and mediates the activation of c-fos gene promoter. HB-EGF enhanced the mRNA expression of tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) mRNA. However, the enhancement of t-PA and PAI-1 by HB-EGF was less than that by EGF. Heparitinase/chlorate, which digests the heparan sulfate proteoglycan of the endothelial cell surface, restored both t-PA and PAI-1 mRNA levels in response to HB-EGF in a manner similar to that by EGF. HB-EGF at 10 ng/ml developed tube-like structures in type I collagen gel at similar levels to that of EGF at 10 ng/ml, suggesting that HB-EGF is also a potent angiogenic factor in the model system for angiogenesis. The tubulogenesis activity of HB-EGF is discussed in relation to the expression of the t-PA and PAI-1 genes. | lld:pubmed |
| pubmed-article:8609052 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8609052 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8609052 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8609052 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8609052 | pubmed:issn | 0910-5050 | lld:pubmed |
| pubmed-article:8609052 | pubmed:author | pubmed-author:OnoMM | lld:pubmed |
| pubmed-article:8609052 | pubmed:author | pubmed-author:TaniguchiNN | lld:pubmed |
| pubmed-article:8609052 | pubmed:author | pubmed-author:KuwanoMM | lld:pubmed |
| pubmed-article:8609052 | pubmed:author | pubmed-author:KohnoKK | lld:pubmed |
| pubmed-article:8609052 | pubmed:author | pubmed-author:IzumiHH | lld:pubmed |
| pubmed-article:8609052 | pubmed:author | pubmed-author:UshiroSS | lld:pubmed |
| pubmed-article:8609052 | pubmed:author | pubmed-author:HigashiyamaSS | lld:pubmed |
| pubmed-article:8609052 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8609052 | pubmed:volume | 87 | lld:pubmed |
| pubmed-article:8609052 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8609052 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8609052 | pubmed:pagination | 68-77 | lld:pubmed |
| pubmed-article:8609052 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:8609052 | pubmed:meshHeading | pubmed-meshheading:8609052-... | lld:pubmed |
| pubmed-article:8609052 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8609052 | pubmed:articleTitle | Heparin-binding epidermal growth factor-like growth factor: p91 activation induction of plasminogen activator/inhibitor, and tubular morphogenesis in human microvascular endothelial cells. | lld:pubmed |
| pubmed-article:8609052 | pubmed:affiliation | Department of Biochemistry, Kyushu University School of Medicine. | lld:pubmed |
| pubmed-article:8609052 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8609052 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8609052 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8609052 | lld:pubmed |
