| pubmed-article:8606116 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8606116 | lifeskim:mentions | umls-concept:C0030637 | lld:lifeskim |
| pubmed-article:8606116 | lifeskim:mentions | umls-concept:C0449943 | lld:lifeskim |
| pubmed-article:8606116 | lifeskim:mentions | umls-concept:C0682744 | lld:lifeskim |
| pubmed-article:8606116 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:8606116 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8606116 | pubmed:dateCreated | 1996-5-23 | lld:pubmed |
| pubmed-article:8606116 | pubmed:abstractText | Neuraminidases produced by 16 strains of Pasteurella multocida (serotypes 1 to 16) were characterized by molecular weight, substrate specificity, and antigenic identity. After growth in a chemically defined medium, stage I (lyophilized) culture supernatants were assayed for activity with N-acetylneuramin lactose, human alpha-1-acid glycoprotein, fetuin, colominic acid, and bovine submaxillary mucin. Neuraminidase produced by P. multocida A:3 was purified by a combination of salt fractionation, ion-exchange chromatography on DEAE-Sephacel, and gel filtration on Sephadex G-200. Purified P. multocida A:3 neuraminidase was employed to immunize rabbits, and the resulting antiserum reduced the activity of the P. multocida A:3 enzyme by 40.3%. This antiserum also reduced the activities of the neuraminidases produced by other serotypes by between 30.8 and 59.6%. Molecular weight estimates of the neuraminidases produced by the various serotypes were obtained by gel filtration chromatography on Sephadex G-200. Each of the 16 serotypes examined produced a neuraminidase with a molecular weight of approximately 500,000. In addition, all 16 high-molecular-weight neuraminidases showed similar substrate specificities. On the basis of these data, it appears that the high-molecular-weight neuraminidases produced by different P. multocida serotypes are quite similar. | lld:pubmed |
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| pubmed-article:8606116 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8606116 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8606116 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8606116 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8606116 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8606116 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8606116 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8606116 | pubmed:issn | 0019-9567 | lld:pubmed |
| pubmed-article:8606116 | pubmed:author | pubmed-author:StrausD CDC | lld:pubmed |
| pubmed-article:8606116 | pubmed:author | pubmed-author:PurdyC WCW | lld:pubmed |
| pubmed-article:8606116 | pubmed:author | pubmed-author:JolleyW LWL | lld:pubmed |
| pubmed-article:8606116 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8606116 | pubmed:volume | 64 | lld:pubmed |
| pubmed-article:8606116 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8606116 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8606116 | pubmed:pagination | 1446-9 | lld:pubmed |
| pubmed-article:8606116 | pubmed:dateRevised | 2010-9-13 | lld:pubmed |
| pubmed-article:8606116 | pubmed:meshHeading | pubmed-meshheading:8606116-... | lld:pubmed |
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| pubmed-article:8606116 | pubmed:meshHeading | pubmed-meshheading:8606116-... | lld:pubmed |
| pubmed-article:8606116 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8606116 | pubmed:articleTitle | Characterization of neuraminidases produced by various serotypes of Pasteurella multocida. | lld:pubmed |
| pubmed-article:8606116 | pubmed:affiliation | Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, Lubbock, Texas 79430, USA. | lld:pubmed |
| pubmed-article:8606116 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8606116 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8606116 | lld:pubmed |
