pubmed-article:8600454 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8600454 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
pubmed-article:8600454 | lifeskim:mentions | umls-concept:C0024660 | lld:lifeskim |
pubmed-article:8600454 | lifeskim:mentions | umls-concept:C0147543 | lld:lifeskim |
pubmed-article:8600454 | lifeskim:mentions | umls-concept:C1148930 | lld:lifeskim |
pubmed-article:8600454 | pubmed:issue | 5 | lld:pubmed |
pubmed-article:8600454 | pubmed:dateCreated | 1996-5-1 | lld:pubmed |
pubmed-article:8600454 | pubmed:abstractText | Nuclear pre-mRNA splicing requires ATP at several steps from spliceosome assembly to product release. Here, we demonstrate that an integral component of the 20S U5 snRNP is an RNA-dependent ATPase. The ATPase activity of 20S U5 and 25S [U4/U6.U5] snRNPs purified by glycerol gradient centrifugation is strongly stimulated by homopolymeric RNA but not ssDNA. Purified 12S Ul and U2 snRNPs do not exhibit ATPase activity. Moreover, the U5-associated NTPase specifically hydrolyzes ATP and dATP. The additional purification of 20S U5 snRNPs by Mono Q chromatography does not affect the efficiency of ATP hydrolysis. Both U5 and tri-snRNPs bind ATP stoichiometrically in an RNA-independent manner. A candidate ATPase was identified by UV-irradiation of purified snRNPs with radiolabeled ATP. In the presence of homopolymeric RNA, the 200 kDa U5-specific protein is the major crosslinked protein, even in Mono Q-purified U5 snRNPs. The correlation between RNA-dependent ATPase activity in the U5 snRNP and the RNA-dependent onset of this crosslink strongly suggests that the 200 kDa protein is an RNA-dependent ATPase. Furthermore, both the formation of the crosslink and ATPase activity appear with a similar substrate specificity for ATP. | lld:pubmed |
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pubmed-article:8600454 | pubmed:language | eng | lld:pubmed |
pubmed-article:8600454 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8600454 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:8600454 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8600454 | pubmed:month | Mar | lld:pubmed |
pubmed-article:8600454 | pubmed:issn | 0305-1048 | lld:pubmed |
pubmed-article:8600454 | pubmed:author | pubmed-author:LührmannRR | lld:pubmed |
pubmed-article:8600454 | pubmed:author | pubmed-author:LauberJJ | lld:pubmed |
pubmed-article:8600454 | pubmed:author | pubmed-author:LaggerbauerBB | lld:pubmed |
pubmed-article:8600454 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8600454 | pubmed:day | 1 | lld:pubmed |
pubmed-article:8600454 | pubmed:volume | 24 | lld:pubmed |
pubmed-article:8600454 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8600454 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8600454 | pubmed:pagination | 868-75 | lld:pubmed |
pubmed-article:8600454 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
pubmed-article:8600454 | pubmed:meshHeading | pubmed-meshheading:8600454-... | lld:pubmed |
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pubmed-article:8600454 | pubmed:meshHeading | pubmed-meshheading:8600454-... | lld:pubmed |
pubmed-article:8600454 | pubmed:year | 1996 | lld:pubmed |
pubmed-article:8600454 | pubmed:articleTitle | Identification of an RNA-dependent ATPase activity in mammalian U5 snRNPs. | lld:pubmed |
pubmed-article:8600454 | pubmed:affiliation | Institut für Molekularbiologie und Tumorforschung, Marburg, Germany. | lld:pubmed |
pubmed-article:8600454 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:8600454 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
entrez-gene:23020 | entrezgene:pubmed | pubmed-article:8600454 | lld:entrezgene |
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