| pubmed-article:8596023 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8596023 | lifeskim:mentions | umls-concept:C0003315 | lld:lifeskim |
| pubmed-article:8596023 | lifeskim:mentions | umls-concept:C0123759 | lld:lifeskim |
| pubmed-article:8596023 | lifeskim:mentions | umls-concept:C0018894 | lld:lifeskim |
| pubmed-article:8596023 | lifeskim:mentions | umls-concept:C0009013 | lld:lifeskim |
| pubmed-article:8596023 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:8596023 | lifeskim:mentions | umls-concept:C1514485 | lld:lifeskim |
| pubmed-article:8596023 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:8596023 | pubmed:dateCreated | 1996-4-15 | lld:pubmed |
| pubmed-article:8596023 | pubmed:abstractText | We investigated the role of IL-12 in proliferation of various Th cell clones (class II-alloreactive (4-86 and 4-55) and keyhole limpet hemocyanin + self I-Ek-reactive (9-16)) following stimulation with Ag on APCs. These clones proliferated in response to stimulation with rIL-2, rIL-12, or Ag/APC. The proliferation induced by Ag/APC stimulation was not affected by anti-IL-2 Ab but was markedly inhibited by anti-IL-12 Abs. Consistent with this finding was the absence of detectable IL-2 activity in culture supernatants 12 to 48 h after Ag/APC stimulation, and the detection of significant levels of IL-12 in an Ab-capture bioassay. IL-12 was produced within 12 h after Ag/APC stimulation, reaching a peak after 18 to 24 h. The production of IL-12 in cultures of Th clones and APC contrasted with the production of IL-2 but not IL-12 upon allostimulation of primary T cells and the inhibition of their proliferation exclusively by anti-IL-2 Abs. Analysis of the expression of IL-12-binding sites on Th cells revealed low levels of IL-12 receptors in resting Th clones but high IL-12R levels 2 to 3 days after Ag/APC stimulation, declining gradually thereafter. The changes in IL-12R expression levels correlated closely with the IL-12 responsiveness of Th populations at various times after Ag/APC stimulation; Th populations obtained 3 and 10 days after Ag/APC stimulation exhibited very high and weak or marginal responsiveness to rIL-12, respectively, whereas the responses to rIL-2 were comparable in both Th populations. These results indicate that the Ag/APC-stimulated proliferation of terminally differentiated Th clones, in contrast to naive T cells, depends on the production of IL-12 by APC and on the simultaneous up-regulation of IL-12R on Th cells rather than on an IL-2 autocrine mechanism. | lld:pubmed |
| pubmed-article:8596023 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8596023 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8596023 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8596023 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8596023 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:8596023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8596023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8596023 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8596023 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8596023 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:8596023 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:KEYY | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:KobayashiMM | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:FujiwaraHH | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:IwataHH | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:YamadaSS | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:HamaokaTT | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:LIT JTJ | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:WysockaMM | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:TrinchieriGG | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:TakenakaHH | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:MaruoSS | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:Toyo-okaKK | lld:pubmed |
| pubmed-article:8596023 | pubmed:author | pubmed-author:Oh-horaMM | lld:pubmed |
| pubmed-article:8596023 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8596023 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:8596023 | pubmed:volume | 156 | lld:pubmed |
| pubmed-article:8596023 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8596023 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8596023 | pubmed:pagination | 1748-55 | lld:pubmed |
| pubmed-article:8596023 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
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| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
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| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:meshHeading | pubmed-meshheading:8596023-... | lld:pubmed |
| pubmed-article:8596023 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8596023 | pubmed:articleTitle | IL-12 produced by antigen-presenting cells induces IL-2-independent proliferation of T helper cell clones. | lld:pubmed |
| pubmed-article:8596023 | pubmed:affiliation | Biomedical Research Center, Osaka University Medical School, Japan. | lld:pubmed |
| pubmed-article:8596023 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8596023 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8596023 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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