| pubmed-article:8588344 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8588344 | lifeskim:mentions | umls-concept:C0521009 | lld:lifeskim |
| pubmed-article:8588344 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:8588344 | lifeskim:mentions | umls-concept:C0036536 | lld:lifeskim |
| pubmed-article:8588344 | lifeskim:mentions | umls-concept:C0021760 | lld:lifeskim |
| pubmed-article:8588344 | lifeskim:mentions | umls-concept:C0036537 | lld:lifeskim |
| pubmed-article:8588344 | lifeskim:mentions | umls-concept:C0085236 | lld:lifeskim |
| pubmed-article:8588344 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:8588344 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:8588344 | pubmed:dateCreated | 1996-3-27 | lld:pubmed |
| pubmed-article:8588344 | pubmed:abstractText | Interleukin-6 (IL-6) is a pluripotent cytokine that may play a role in pulmonary defense against bacterial pathogens. We have quantitated the response of bovine alveolar macrophages (bAM) to bacterial lipopolysaccharide (LPS; E. coli 055: B5) in vitro using the IL-6 sensitive 7TD1 cell line. Bacteria LPS in the absence of serum induced IL-6 secretion from bAM (1 x 10(6) ml-1) over a range of LPS concentrations from 10 ng ml-1 to 10 micrograms ml-1. This resulted in IL-6 levels ranging from approximately 5 to over 200 U ml-1.IL-6 secretion by from approximately 5 to over 200 U ml-1.IL-6 secretion by LPS-stimulated bAM was increased by 24 h poststimulation, and continued to increase up to 72 h after stimulation. Fetal bovine serum (FBS, 1% vol/vol; 320 micrograms ml-1) enhanced IL-6 secretion from macrophages in the presence of LPS by approximately 10-fold compared with LPS alone. A bovine serum fraction (1 microgram ml-1 protein) prepared using ion-exchange chromatography also markedly enhanced IL-6 secretion versus LPS alone. The stimulatory effect of IL-6-like activity in the bAM supernatants was neutralized by an anti-human IL-6 polyclonal antibody. Northern blot analysis revealed increased IL-6 mRNA at 2 h poststimulation with LPS + FBS, peak levels at 4 h, and levels were decreased by 6 h poststimulation. Results suggest that IL-6 is secreted by bovine alveolar macrophages, and that bacterial LPS and serum components synergize to produce this response. | lld:pubmed |
| pubmed-article:8588344 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8588344 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8588344 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8588344 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8588344 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8588344 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8588344 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8588344 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8588344 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8588344 | pubmed:issn | 0165-2427 | lld:pubmed |
| pubmed-article:8588344 | pubmed:author | pubmed-author:DíezVV | lld:pubmed |
| pubmed-article:8588344 | pubmed:author | pubmed-author:SlausonD ODO | lld:pubmed |
| pubmed-article:8588344 | pubmed:author | pubmed-author:MillerM SMS | lld:pubmed |
| pubmed-article:8588344 | pubmed:author | pubmed-author:CarterC DCD | lld:pubmed |
| pubmed-article:8588344 | pubmed:author | pubmed-author:YanoRR | lld:pubmed |
| pubmed-article:8588344 | pubmed:author | pubmed-author:BochslerP NPN | lld:pubmed |
| pubmed-article:8588344 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8588344 | pubmed:volume | 49 | lld:pubmed |
| pubmed-article:8588344 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8588344 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8588344 | pubmed:pagination | 51-60 | lld:pubmed |
| pubmed-article:8588344 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8588344 | pubmed:meshHeading | pubmed-meshheading:8588344-... | lld:pubmed |
| pubmed-article:8588344 | pubmed:meshHeading | pubmed-meshheading:8588344-... | lld:pubmed |
| pubmed-article:8588344 | pubmed:meshHeading | pubmed-meshheading:8588344-... | lld:pubmed |
| pubmed-article:8588344 | pubmed:meshHeading | pubmed-meshheading:8588344-... | lld:pubmed |
| pubmed-article:8588344 | pubmed:meshHeading | pubmed-meshheading:8588344-... | lld:pubmed |
| pubmed-article:8588344 | pubmed:meshHeading | pubmed-meshheading:8588344-... | lld:pubmed |
| pubmed-article:8588344 | pubmed:meshHeading | pubmed-meshheading:8588344-... | lld:pubmed |
| pubmed-article:8588344 | pubmed:meshHeading | pubmed-meshheading:8588344-... | lld:pubmed |
| pubmed-article:8588344 | pubmed:meshHeading | pubmed-meshheading:8588344-... | lld:pubmed |
| pubmed-article:8588344 | pubmed:meshHeading | pubmed-meshheading:8588344-... | lld:pubmed |
| pubmed-article:8588344 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:8588344 | pubmed:articleTitle | Interleukin-6 secretion by bacterial lipopolysaccharide-stimulated bovine alveolar macrophages in vitro. | lld:pubmed |
| pubmed-article:8588344 | pubmed:affiliation | Department of Pathology, College of Veterinary Medicine, University of Tennessee, Knoxville 37901, USA. | lld:pubmed |
| pubmed-article:8588344 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8588344 | pubmed:publicationType | In Vitro | lld:pubmed |
