| pubmed-article:8586703 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8586703 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:8586703 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:8586703 | lifeskim:mentions | umls-concept:C0184661 | lld:lifeskim |
| pubmed-article:8586703 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:8586703 | lifeskim:mentions | umls-concept:C1948027 | lld:lifeskim |
| pubmed-article:8586703 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:8586703 | lifeskim:mentions | umls-concept:C1709793 | lld:lifeskim |
| pubmed-article:8586703 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:8586703 | pubmed:dateCreated | 1996-3-27 | lld:pubmed |
| pubmed-article:8586703 | pubmed:abstractText | Two quantitative PCR methods with our nonisotopic enzyme-linked oligosorbent assay (ELOSA) in microtiter plate format were developed for quantitation of human immunodeficiency virus type 1 (HIV-1). Quantitative competitive PCR (QC-PCR) was based on the coamplification of the wild-type nef region with a mimic competitive nef gene template carrying mutations in the capture region. Correlation of wild-type HIV-1 nef DNA to mimic template copy number permitted quantitation of HIV-1 copy numbers in the range of 20 to 2,000 copies per micrograms of DNA. Internally controlled PCR (IC-PCR) was based on coamplification of the nef region and the ras gene as an internal endogenous standard. Correlation to known amounts of HIV-1 DNA permitted quantitation by IC-PCR of HIV-1 copy numbers in the range of 10 to 2,000 copies per microgram of DNA. QC- and IC-PCR-ELOSA were performed on a panel of 53 seropositive patients and 12 seronegative controls. The methods showed similar coefficients of variation below 24%. Quantitations by QC- and IC-PCR-ELOSA were identical for 77% of patient samples. The copy level ranged between 443 +/- 156 and 21,453 +/- 13,511 copies per 10(5) CD4 cells for asymptomatic and AIDS patients, respectively. The simplicity and reliability of QC- and IC-PCR-ELOSA methods make them appropriate for routine laboratory use in the quantitation of viral and bacterial DNAs. | lld:pubmed |
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| pubmed-article:8586703 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8586703 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8586703 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8586703 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8586703 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8586703 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:8586703 | pubmed:author | pubmed-author:TouraineJ LJL | lld:pubmed |
| pubmed-article:8586703 | pubmed:author | pubmed-author:TropDD | lld:pubmed |
| pubmed-article:8586703 | pubmed:author | pubmed-author:LeesOO | lld:pubmed |
| pubmed-article:8586703 | pubmed:author | pubmed-author:MallerDD | lld:pubmed |
| pubmed-article:8586703 | pubmed:author | pubmed-author:MandrandBB | lld:pubmed |
| pubmed-article:8586703 | pubmed:author | pubmed-author:LivrozetJ MJM | lld:pubmed |
| pubmed-article:8586703 | pubmed:author | pubmed-author:HebrardCC | lld:pubmed |
| pubmed-article:8586703 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8586703 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:8586703 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8586703 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8586703 | pubmed:pagination | 3201-8 | lld:pubmed |
| pubmed-article:8586703 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8586703 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:8586703 | pubmed:articleTitle | Quantitation of human immunodeficiency virus type 1 DNA by two PCR procedures coupled with enzyme-linked oligosorbent assay. | lld:pubmed |
| pubmed-article:8586703 | pubmed:affiliation | Unité Mixte de Recherche 103 Centre National de la Recherche Scientifique-bioMérieux, Ecole Normale Supérieure de Lyon, France. | lld:pubmed |
| pubmed-article:8586703 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8586703 | pubmed:publicationType | Comparative Study | lld:pubmed |
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