| pubmed-article:8577939 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C0008109 | lld:lifeskim |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C0021670 | lld:lifeskim |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C0243192 | lld:lifeskim |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C0002482 | lld:lifeskim |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C0030956 | lld:lifeskim |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C0060993 | lld:lifeskim |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C0441472 | lld:lifeskim |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C0231491 | lld:lifeskim |
| pubmed-article:8577939 | lifeskim:mentions | umls-concept:C0972293 | lld:lifeskim |
| pubmed-article:8577939 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:8577939 | pubmed:dateCreated | 1996-3-14 | lld:pubmed |
| pubmed-article:8577939 | pubmed:abstractText | The chimeric peptide M35 [galanin(1-13)-bradykinin(2-9) amide] is a high-affinity galanin receptor ligand acting as a galanin receptor antagonist in the rat spinal cord, rat hippocampus and isolated mouse pancreatic islets. We have radiolabelled M35 and performed equilibrium binding studies with [125I]M35 on the rat pancreatic beta-cell line Rin m 5F, whereby we show the existence of high-affinity binding site (KD = 0.9 +/- 0.1 nM) with a Bmax of 72 +/- 3 fmol/mg protein. Galanin displaces [125I]M35 with the same affinity (KD = 1 nM) as it displaces [125I]galanin. Displacement of [125I]galanin by M35 from Rin m 5F cell membranes shows the presence of two binding sites for M35 with KD-values of 0.3 +/- 0.1 nM and 0.52 +/- 0.03 microM, respectively. The GTP- and pertussis toxin-sensitivity of M35 binding to Rin m 5F membranes shows that binding of [125I]M35 is almost completely abolished by the presence of GTP or after pertussis toxin treatment of the cells, indicating an agonist-like binding of M35 to the galanin receptors. M35 has a dual effect on the galanin mediated inhibition of forskolin stimulated cyclic AMP production in Rin m 5F cells: at low concentrations M35 antagonises the effect of galanin, whereas at concentrations above 10 nM M35 acts as a galanin receptor agonist. These agonist-like effects of galanin and M35 are not additive, thus the mixed agonist/antagonist properties arise from the chimeric nature of M35[galanin(1-13)-bradykinin(2-9)amide] acting solely at galanin receptors. | lld:pubmed |
| pubmed-article:8577939 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8577939 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8577939 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8577939 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8577939 | pubmed:issn | 0167-0115 | lld:pubmed |
| pubmed-article:8577939 | pubmed:author | pubmed-author:BartfaiTT | lld:pubmed |
| pubmed-article:8577939 | pubmed:author | pubmed-author:LangelUU | lld:pubmed |
| pubmed-article:8577939 | pubmed:author | pubmed-author:BourneJJ | lld:pubmed |
| pubmed-article:8577939 | pubmed:author | pubmed-author:KaseMM | lld:pubmed |
| pubmed-article:8577939 | pubmed:author | pubmed-author:BertholdMM | lld:pubmed |
| pubmed-article:8577939 | pubmed:author | pubmed-author:AndellSS | lld:pubmed |
| pubmed-article:8577939 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8577939 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:8577939 | pubmed:volume | 59 | lld:pubmed |
| pubmed-article:8577939 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8577939 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8577939 | pubmed:pagination | 341-8 | lld:pubmed |
| pubmed-article:8577939 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:8577939 | pubmed:meshHeading | pubmed-meshheading:8577939-... | lld:pubmed |
| pubmed-article:8577939 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:8577939 | pubmed:articleTitle | Binding and agonist/antagonist actions of M35, galanin(1-13)-bradykinin(2-9)amide chimeric peptide, in Rin m 5F insulinoma cells. | lld:pubmed |
| pubmed-article:8577939 | pubmed:affiliation | Department of Neurochemistry and Neurotoxicology, Arrhenius Laboratories, Stockholm University, Sweden. | lld:pubmed |
| pubmed-article:8577939 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8577939 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8577939 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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