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pubmed-article:8573076pubmed:abstractTextOur previous studies [Chao (1992) Biochem. J. 282, 203-207; C.C.-K. Chao, unpublished work] has suggested a correlation between the levels of constitutive UV-damaged-DNA-recognitionproteins (UVDRP) and cellular DNA repair in different cell types. In the present study, UVDRP were induced in F9 and NIH3T3 cells by 0.1 microM retinoic acid (RA) and 1 mM dibutyryl cyclic AMP (dbcAMP), which is sufficient to induce differentiation in murine F9 stem cells. The induction of UVDRP in F9 and NIH3T3 cells was optimized after 6 and 2 days incubation with RA/dbcAMP respectively. Since NIH3T3 cells were not induced to differentiate by RA/dbcAMP, the upregulation of the UVDRP in mammalian cells would thus seem not to be mediated directly by differentiation. Using a plasmid re-activation assay to estimate DNA repair, we did not find a correlation between DNA repair and UVDRP in RA/dbcAMP-treated cells. The results suggest that UVDRP may have a function other than, or in addition to, its role in DNA repair.lld:pubmed
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pubmed-article:8573076pubmed:authorpubmed-author:HuangS LSLlld:pubmed
pubmed-article:8573076pubmed:authorpubmed-author:Lin-ChaoSSlld:pubmed
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pubmed-article:8573076pubmed:articleTitleInduction, not associated with host-cell re-activation of damaged plasmid DNA, of damaged-DNA-recognition proteins by retinoic acid and dibutyryl cyclic AMP in mammalian cells.lld:pubmed
pubmed-article:8573076pubmed:affiliationDepartment of Biochemistry, Chang Gung Medical College, Taoyuan, Taiwan, Republic of China.lld:pubmed
pubmed-article:8573076pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:8573076pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed