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pubmed-article:8560388pubmed:abstractTextWe recently demonstrated that rat pulmonary artery smooth muscle (RPASM) generates maximal nitric oxide (NO) when exposed to inflammatory cytokines, such as tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma. Our hypothesis is that NO produced by cytokine-stimulated RPASM has local cytotoxic effects on endothelium. Accordingly, we designed a pulmonary smooth muscle and endothelial coculture experiment in which the effects of NO on endothelium can be distinguished from the direct effects of cytokines.lld:pubmed
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pubmed-article:8560388pubmed:articleTitleNitric oxide produced by cytokine-activated pulmonary artery smooth muscle cells is cytotoxic to cocultured endothelium.lld:pubmed
pubmed-article:8560388pubmed:affiliationDepartment of Surgery, University of North Carolina at Chapel Hill, USA.lld:pubmed
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