| pubmed-article:8554506 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8554506 | lifeskim:mentions | umls-concept:C0017742 | lld:lifeskim |
| pubmed-article:8554506 | lifeskim:mentions | umls-concept:C0205245 | lld:lifeskim |
| pubmed-article:8554506 | lifeskim:mentions | umls-concept:C0041217 | lld:lifeskim |
| pubmed-article:8554506 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8554506 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:8554506 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:8554506 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:8554506 | pubmed:dateCreated | 1996-2-20 | lld:pubmed |
| pubmed-article:8554506 | pubmed:abstractText | The gene encoding THT2, one of two hexose-transporter isoforms present in Trypanosoma brucei, has been expressed in both Xenopus laevis oocytes and a stably transfected line of Chinese hamster ovary (CHO) cells. The heterologously expressed gene encodes a protein with pharmacological and kinetic parameters similar to those of the hexose transporter measured in procyclic-culture-form trypanosomes. The substrate recognition of the THT2 transporter differed from that of the THT1 isoform, which is expressed only in bloodstream forms, in that: (i) it has a relatively high affinity for substrate with a Km of 59 microM for 2-deoxy-D-glucose (2-DOG) and a similar high affinity for D-glucose (compared with Km of 0.5 mM for 2-DOG in bloodstream forms); (ii) the affinity for 6-deoxy-D-glucose (6-DOG) is two orders of magnitude lower than that for D-glucose, whereas the bloodstream-form transporter recognizes D-glucose and its 6-DOG analogue with similar affinity; (iii) the bloodstream-form transporter, but not THT2, recognizes 3-fluoro-3-deoxy-D-glucose. D-Fructose-transport capacity and insensitivity to D-galactose was also found in THT2-expressing CHO cells and procyclic trypanosomes. We conclude from these cumulative results that the THT2 gene encodes the transporter responsible for hexose transport in procyclic trypanosomes. The transport of 2-DOG in procyclic organisms was inhibited by both the protonophore, carbonyl cyanide 4-trifluoromethoxy phenylhydrazone (FCCP), and KCN, suggesting a requirement for a protonmotive force. However, sensitivity to these reagents depended on the external substrate concentration, with uptake being unaffected at substrate concentrations higher than 2 mM. THT2 expressed in CHO cells behaved as a facilitated transporter, and was unaffected by FCCP or KCN over the whole substrate concentration range tested. | lld:pubmed |
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| pubmed-article:8554506 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8554506 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8554506 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8554506 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8554506 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8554506 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:8554506 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:8554506 | pubmed:author | pubmed-author:BaltzTT | lld:pubmed |
| pubmed-article:8554506 | pubmed:author | pubmed-author:BringaudFF | lld:pubmed |
| pubmed-article:8554506 | pubmed:author | pubmed-author:SeyfangAA | lld:pubmed |
| pubmed-article:8554506 | pubmed:author | pubmed-author:BarrettM PMP | lld:pubmed |
| pubmed-article:8554506 | pubmed:author | pubmed-author:TetaudEE | lld:pubmed |
| pubmed-article:8554506 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8554506 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8554506 | pubmed:volume | 312 ( Pt 3) | lld:pubmed |
| pubmed-article:8554506 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8554506 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8554506 | pubmed:pagination | 687-91 | lld:pubmed |
| pubmed-article:8554506 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8554506 | pubmed:meshHeading | pubmed-meshheading:8554506-... | lld:pubmed |
| pubmed-article:8554506 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:8554506 | pubmed:articleTitle | Functional expression and characterization of the Trypanosoma brucei procyclic glucose transporter, THT2. | lld:pubmed |
| pubmed-article:8554506 | pubmed:affiliation | Université de Bordeaux II, France. | lld:pubmed |
| pubmed-article:8554506 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8554506 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:3662271 | entrezgene:pubmed | pubmed-article:8554506 | lld:entrezgene |
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