| pubmed-article:8547219 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8547219 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:8547219 | lifeskim:mentions | umls-concept:C0017636 | lld:lifeskim |
| pubmed-article:8547219 | lifeskim:mentions | umls-concept:C0007595 | lld:lifeskim |
| pubmed-article:8547219 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:8547219 | lifeskim:mentions | umls-concept:C0332161 | lld:lifeskim |
| pubmed-article:8547219 | lifeskim:mentions | umls-concept:C0079419 | lld:lifeskim |
| pubmed-article:8547219 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:8547219 | lifeskim:mentions | umls-concept:C0249197 | lld:lifeskim |
| pubmed-article:8547219 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:8547219 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:8547219 | pubmed:dateCreated | 1996-2-20 | lld:pubmed |
| pubmed-article:8547219 | pubmed:abstractText | The WAF1/Cip1 protein is an important regulator at the G1 checkpoint in the cell cycle. The WAF1/Cip1 protein binds to the cyclin-dependent kinase complexes and inhibits the kinase activity that is required for cell cycle progression. We investigated the expression of WAF1/Cip1 protein in 14 glioblastoma cell lines and found that WAF1/Cip1 expression was detectable in many of the cell lines, even when mutant p53 was present. We also showed that WAF1/Cip1 protein level was very low in LN-Z308 cells that do not express endogenous p53. Transfection of the wild-type p53 into this cell line activated WAF1/Cip1 expression and inhibited cell growth. In contrast, transfection of the p53 mutant 248Trp failed to activate WAF1/Cip1 expression. Transfection of WAF1/Cip1 alone also inhibited LN-Z308 cell proliferation. However, cotransfection of the p53 mutant 248Trp with WAF1/Cip1 attenuated the growth-suppression effect of WAF1/Cip1. Our analysis with Western blot showed that the levels of cyclin E increased in cells transfected with p53 mutants. We conclude that p53 mutants may counter the negative regulators, such as WAF1/Cip1, by the elevation of positive cell cycle regulators, and the presence of WAF1/Cip1 in tumor cells is not sufficient for growth inhibition. | lld:pubmed |
| pubmed-article:8547219 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8547219 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8547219 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8547219 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8547219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8547219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8547219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8547219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8547219 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8547219 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8547219 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8547219 | pubmed:issn | 1044-9523 | lld:pubmed |
| pubmed-article:8547219 | pubmed:author | pubmed-author:KobayashiTT | lld:pubmed |
| pubmed-article:8547219 | pubmed:author | pubmed-author:LevinV AVA | lld:pubmed |
| pubmed-article:8547219 | pubmed:author | pubmed-author:LiHH | lld:pubmed |
| pubmed-article:8547219 | pubmed:author | pubmed-author:LangfordL ALA | lld:pubmed |
| pubmed-article:8547219 | pubmed:author | pubmed-author:BrunerJ MJM | lld:pubmed |
| pubmed-article:8547219 | pubmed:author | pubmed-author:ZhangWW | lld:pubmed |
| pubmed-article:8547219 | pubmed:author | pubmed-author:KyritsisA PAP | lld:pubmed |
| pubmed-article:8547219 | pubmed:author | pubmed-author:JungJ MJM | lld:pubmed |
| pubmed-article:8547219 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8547219 | pubmed:volume | 6 | lld:pubmed |
| pubmed-article:8547219 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8547219 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8547219 | pubmed:pagination | 909-13 | lld:pubmed |
| pubmed-article:8547219 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:meshHeading | pubmed-meshheading:8547219-... | lld:pubmed |
| pubmed-article:8547219 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:8547219 | pubmed:articleTitle | Inhibition of human glioblastoma cell growth by WAF1/Cip1 can be attenuated by mutant p53. | lld:pubmed |
| pubmed-article:8547219 | pubmed:affiliation | Department of Neuro-Oncology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA. | lld:pubmed |
| pubmed-article:8547219 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8547219 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8547219 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
