pubmed-article:8532524 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8532524 | lifeskim:mentions | umls-concept:C0027923 | lld:lifeskim |
pubmed-article:8532524 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
pubmed-article:8532524 | lifeskim:mentions | umls-concept:C0036002 | lld:lifeskim |
pubmed-article:8532524 | lifeskim:mentions | umls-concept:C0376452 | lld:lifeskim |
pubmed-article:8532524 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
pubmed-article:8532524 | lifeskim:mentions | umls-concept:C0301630 | lld:lifeskim |
pubmed-article:8532524 | lifeskim:mentions | umls-concept:C1524003 | lld:lifeskim |
pubmed-article:8532524 | lifeskim:mentions | umls-concept:C0392760 | lld:lifeskim |
pubmed-article:8532524 | pubmed:issue | 23 | lld:pubmed |
pubmed-article:8532524 | pubmed:dateCreated | 1996-2-1 | lld:pubmed |
pubmed-article:8532524 | pubmed:abstractText | A temperature-sensitive methionine auxotroph of Neurospora crassa was found in a collection of conditional mutants and shown to be deficient in DNA methylation when grown under semipermissive conditions. The defective gene was identified as met-3, which encodes cystathionine-gamma-synthase. We explored the possibility that the methylation defect results from deficiency of S-adenosylmethionine (SAM), the presumptive methyl group donor. Methionine starvation of mutants from each of nine complementation groups in the methionine (met) pathway (met-1, met-2, met-3, met-5, met-6, met-8, met-9, met-10 and for) resulted in decreased DNA methylation while amino acid starvation, per se, did not. In most of the strains, including wild-type, intracellular SAM peaked during rapid growth (12-18 h after inoculation), whereas DNA methylation continued to increase. In met mutants starved for methionine, SAM levels were most reduced (3-11-fold) during rapid growth while the greatest reduction in DNA methylation levels occurred later. Addition of 3 mM methionine to cultures of met or cysteine-requiring (cys) mutants resulted in 5-28-fold increases in SAM, compared with wild-type, at a time when DNA methylation was reduced approximately 40%, suggesting that the decreased methylation during rapid growth in Neurospora is not due to limiting SAM. DNA methylation continued to increase in a cys-3 mutant that had stopped growing due to methionine starvation, suggesting that methylation is not obligatorily coupled to DNA replication in Neurospora. | lld:pubmed |
pubmed-article:8532524 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:language | eng | lld:pubmed |
pubmed-article:8532524 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:8532524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8532524 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8532524 | pubmed:month | Dec | lld:pubmed |
pubmed-article:8532524 | pubmed:issn | 0305-1048 | lld:pubmed |
pubmed-article:8532524 | pubmed:author | pubmed-author:RobertsC JCJ | lld:pubmed |
pubmed-article:8532524 | pubmed:author | pubmed-author:SelkerE UEU | lld:pubmed |
pubmed-article:8532524 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8532524 | pubmed:day | 11 | lld:pubmed |
pubmed-article:8532524 | pubmed:volume | 23 | lld:pubmed |
pubmed-article:8532524 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8532524 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8532524 | pubmed:pagination | 4818-26 | lld:pubmed |
pubmed-article:8532524 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
pubmed-article:8532524 | pubmed:meshHeading | pubmed-meshheading:8532524-... | lld:pubmed |
pubmed-article:8532524 | pubmed:meshHeading | pubmed-meshheading:8532524-... | lld:pubmed |
pubmed-article:8532524 | pubmed:meshHeading | pubmed-meshheading:8532524-... | lld:pubmed |
pubmed-article:8532524 | pubmed:meshHeading | pubmed-meshheading:8532524-... | lld:pubmed |
pubmed-article:8532524 | pubmed:meshHeading | pubmed-meshheading:8532524-... | lld:pubmed |
pubmed-article:8532524 | pubmed:meshHeading | pubmed-meshheading:8532524-... | lld:pubmed |
pubmed-article:8532524 | pubmed:meshHeading | pubmed-meshheading:8532524-... | lld:pubmed |
pubmed-article:8532524 | pubmed:meshHeading | pubmed-meshheading:8532524-... | lld:pubmed |
pubmed-article:8532524 | pubmed:meshHeading | pubmed-meshheading:8532524-... | lld:pubmed |
pubmed-article:8532524 | pubmed:meshHeading | pubmed-meshheading:8532524-... | lld:pubmed |
pubmed-article:8532524 | pubmed:year | 1995 | lld:pubmed |
pubmed-article:8532524 | pubmed:articleTitle | Mutations affecting the biosynthesis of S-adenosylmethionine cause reduction of DNA methylation in Neurospora crassa. | lld:pubmed |
pubmed-article:8532524 | pubmed:affiliation | Institute of Molecular Biology, University of Oregon, Eugene 97403, USA. | lld:pubmed |
pubmed-article:8532524 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:8532524 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
pubmed-article:8532524 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8532524 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8532524 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8532524 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8532524 | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8532524 | lld:pubmed |