| pubmed-article:8510224 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8510224 | lifeskim:mentions | umls-concept:C0019169 | lld:lifeskim |
| pubmed-article:8510224 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:8510224 | lifeskim:mentions | umls-concept:C1327616 | lld:lifeskim |
| pubmed-article:8510224 | lifeskim:mentions | umls-concept:C0443803 | lld:lifeskim |
| pubmed-article:8510224 | lifeskim:mentions | umls-concept:C0444669 | lld:lifeskim |
| pubmed-article:8510224 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:8510224 | lifeskim:mentions | umls-concept:C0456378 | lld:lifeskim |
| pubmed-article:8510224 | lifeskim:mentions | umls-concept:C1704222 | lld:lifeskim |
| pubmed-article:8510224 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:8510224 | pubmed:dateCreated | 1993-7-12 | lld:pubmed |
| pubmed-article:8510224 | pubmed:abstractText | Hepatitis B virus, the prototypic member of the Hepadnaviridae, is a small enveloped DNA virus that replicates via reverse transcription. Efficient usage of its compact 3.2-kb genome is exemplified by the pre-C/C gene from which two proteins with largely overlapping primary sequences but distinctly different properties are synthesized: the self-assembling core protein p21c (hepatitis B core antigen [HbcAg]) and the secretory, nonparticulate protein p17e (hepatitis B e antigen [HbeAg]). Mature p17e carries a 10-amino-acid N-terminal extension with a Cys residue (Cys-7). Using transient transfection of a human liver cell line with constructs expressing wild-type p17 or a series of Cys mutants of p17, we show that Cys-7 forms an intramolecular S-S bond to Cys61, which in assembly-competent core proteins is available for intermolecular disulfide bonds between two neighboring subunits. Removal of the Cys-7/Cys61 bond by mutating either residue has differential effects: in the absence of Cys-7, secretion is relatively efficient and independent of Cys61; however, the molecules are exported as homodimers exhibiting both HBe and HBc antigenicity. In the absence of Cys61, the nonpaired Cys-7 interferes with secretion efficiency. The amino acid sequence flanking Cys-7 also contributes to the formation of the proper intramolecular S-S bond. These results suggest that the Cys-7/Cys61 bond imposes on p17e a conformation that is critical for its secretion and distinct biophysical and antigenic properties. This mechanism adds selective disulfide formation to the repertoire of hepatitis B virus for efficient use of its tiny genome. | lld:pubmed |
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| pubmed-article:8510224 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8510224 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8510224 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8510224 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8510224 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:8510224 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:8510224 | pubmed:author | pubmed-author:RiegerAA | lld:pubmed |
| pubmed-article:8510224 | pubmed:author | pubmed-author:NassalMM | lld:pubmed |
| pubmed-article:8510224 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8510224 | pubmed:volume | 67 | lld:pubmed |
| pubmed-article:8510224 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8510224 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8510224 | pubmed:pagination | 4307-15 | lld:pubmed |
| pubmed-article:8510224 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8510224 | pubmed:meshHeading | pubmed-meshheading:8510224-... | lld:pubmed |
| pubmed-article:8510224 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8510224 | pubmed:articleTitle | An intramolecular disulfide bridge between Cys-7 and Cys61 determines the structure of the secretory core gene product (e antigen) of hepatitis B virus. | lld:pubmed |
| pubmed-article:8510224 | pubmed:affiliation | Zentrum für Molekulare Biologie, Universität Heidelberg, Germany. | lld:pubmed |
| pubmed-article:8510224 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8510224 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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