| pubmed-article:8504091 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8504091 | lifeskim:mentions | umls-concept:C0521324 | lld:lifeskim |
| pubmed-article:8504091 | lifeskim:mentions | umls-concept:C0027096 | lld:lifeskim |
| pubmed-article:8504091 | lifeskim:mentions | umls-concept:C1548779 | lld:lifeskim |
| pubmed-article:8504091 | lifeskim:mentions | umls-concept:C1947902 | lld:lifeskim |
| pubmed-article:8504091 | lifeskim:mentions | umls-concept:C2827499 | lld:lifeskim |
| pubmed-article:8504091 | lifeskim:mentions | umls-concept:C0045917 | lld:lifeskim |
| pubmed-article:8504091 | lifeskim:mentions | umls-concept:C0146894 | lld:lifeskim |
| pubmed-article:8504091 | lifeskim:mentions | umls-concept:C1167624 | lld:lifeskim |
| pubmed-article:8504091 | pubmed:issue | 22 | lld:pubmed |
| pubmed-article:8504091 | pubmed:dateCreated | 1993-7-8 | lld:pubmed |
| pubmed-article:8504091 | pubmed:abstractText | The purine binding site of ATP on skeletal muscle myosin has been photoaffinity labeled with 2-azidoadenosine diphosphate (2-N3ADP). 2-N3ADP was stably trapped at the active site (t1/2 approximately 5 days, 0 degree C) by complexation of the two heavy chain reactive thiols (Cys-697 and Cys-707) with Co(III)phenanthroline. Photoincorporation occurred only in the 23-kDa NH2-terminal tryptic fragment of the heavy chain. Extensive serial digestion of photolabeled subfragment 1 of myosin by trypsin and subtilisin yielded a series of labeled peptides which were purified by HPLC. Sequence and radiolabeling analysis of eight photolabeled peptides all indicated that tryptophan-130 was the only labeled residue. This site of labeling confirms earlier photolabeling studies with the non-nucleotide ADP analogue, 2[(4-azido-2-nitrophenyl)-amino]ethyl diphosphate (NANDP), which also labeled Trp-130 [Okamoto, Y., & Yount, R. G. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 1575-1579]. Comparison of the structures of 2-N3ADP and NANDP indicate that their azido groups can be superimposed if both analogues bind to the active site in an extended conformation in a manner analogous to the anti conformation of ATP. | lld:pubmed |
| pubmed-article:8504091 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8504091 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8504091 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8504091 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8504091 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:8504091 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8504091 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:8504091 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:8504091 | pubmed:author | pubmed-author:YountR GRG | lld:pubmed |
| pubmed-article:8504091 | pubmed:author | pubmed-author:KuwayamaHH | lld:pubmed |
| pubmed-article:8504091 | pubmed:author | pubmed-author:GrammerJ CJC | lld:pubmed |
| pubmed-article:8504091 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8504091 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:8504091 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:8504091 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8504091 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8504091 | pubmed:pagination | 5725-32 | lld:pubmed |
| pubmed-article:8504091 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:8504091 | pubmed:meshHeading | pubmed-meshheading:8504091-... | lld:pubmed |
| pubmed-article:8504091 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8504091 | pubmed:articleTitle | Photoaffinity labeling of skeletal myosin with 2-azidoadenosine triphosphate. | lld:pubmed |
| pubmed-article:8504091 | pubmed:affiliation | Department of Biochemistry and Biophysics, Washington State University, Pullman 99164-4660. | lld:pubmed |
| pubmed-article:8504091 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8504091 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8504091 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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