| pubmed-article:8467798 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8467798 | lifeskim:mentions | umls-concept:C0036025 | lld:lifeskim |
| pubmed-article:8467798 | lifeskim:mentions | umls-concept:C0733755 | lld:lifeskim |
| pubmed-article:8467798 | lifeskim:mentions | umls-concept:C2587213 | lld:lifeskim |
| pubmed-article:8467798 | lifeskim:mentions | umls-concept:C1504308 | lld:lifeskim |
| pubmed-article:8467798 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8467798 | pubmed:dateCreated | 1993-5-13 | lld:pubmed |
| pubmed-article:8467798 | pubmed:abstractText | During meiosis, a transient DNA double-strand break (DSB) occurs in the promoter region (positions -200/-185) of the Saccharomyces cerevisiae ARG4 gene and is a likely intermediate in the initiation of meiotic gene conversion events in this region. We report here a functional analysis of the ARG4 DSB based on the study of various deletions in this chromosomal region. We have identified several cis-acting elements located within the -465/+3 region of the ARG4 promoter that control the formation of this DSB. The -465/-317 region includes a transcription terminator and is necessary for a normal amount of ARG4 DSB, but not for its positioning. The -316/-140 region can be replaced by an unrelated DNA sequence where a meiotic DSB then occurs, suggesting that the site of DSB is not sequence-specific, but is positioned at a fixed distance from the adjacent -139/+3 region. Also, in all strains constructed, the amount of meiotic DSB is correlated with the frequency of gene conversion in ARG4, which provides a strong argument for the initiation of gene conversion by a DSB in this region of the yeast genome. | lld:pubmed |
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| pubmed-article:8467798 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8467798 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8467798 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8467798 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8467798 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8467798 | pubmed:issn | 0261-4189 | lld:pubmed |
| pubmed-article:8467798 | pubmed:author | pubmed-author:NicolasAA | lld:pubmed |
| pubmed-article:8467798 | pubmed:author | pubmed-author:de MassyBB | lld:pubmed |
| pubmed-article:8467798 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8467798 | pubmed:volume | 12 | lld:pubmed |
| pubmed-article:8467798 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8467798 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8467798 | pubmed:pagination | 1459-66 | lld:pubmed |
| pubmed-article:8467798 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8467798 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8467798 | pubmed:articleTitle | The control in cis of the position and the amount of the ARG4 meiotic double-strand break of Saccharomyces cerevisiae. | lld:pubmed |
| pubmed-article:8467798 | pubmed:affiliation | Institut de Génétique et Microbiologie, Université Paris-Sud, Orsay, France. | lld:pubmed |
| pubmed-article:8467798 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8467798 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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