| pubmed-article:8458075 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8458075 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:8458075 | lifeskim:mentions | umls-concept:C0086045 | lld:lifeskim |
| pubmed-article:8458075 | lifeskim:mentions | umls-concept:C0391850 | lld:lifeskim |
| pubmed-article:8458075 | lifeskim:mentions | umls-concept:C0175721 | lld:lifeskim |
| pubmed-article:8458075 | lifeskim:mentions | umls-concept:C0044151 | lld:lifeskim |
| pubmed-article:8458075 | lifeskim:mentions | umls-concept:C1664784 | lld:lifeskim |
| pubmed-article:8458075 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8458075 | pubmed:dateCreated | 1993-4-27 | lld:pubmed |
| pubmed-article:8458075 | pubmed:abstractText | New procedures are described for producing brief transients and reversible elevations in [Ca] that can be used to quantitatively control the concentration of cytoplasmic calcium. If the photolabile calcium chelator DM-nitrophen, partially bound to calcium, is exposed to steady illumination, [Ca] can be raised from a few nM to up to 10 microM for durations of 100 ms or longer, depending on light intensity and duration. An association rate of calcium with nitrophen of 1.5 x 10(6) M-1s-1 was estimated from measurements of [Ca] using the fluorescent indicator Fluo-3, and calcium was found to speed the photolysis of nitrophen 2.5-times. Partial photolysis of DM-nitrophen partly loaded with calcium elicits a [Ca] spike of over 100 microM lasting about 1 ms, depending on intensity and duration of the light flash. Simulations of the reactions involved predict changes in Fluo-3 fluorescence measured at high time resolution with a laser scanning confocal microscope. These procedures have been applied in physiological experiments to generate cytoplasmic [Ca] spikes and pulses and study the cellular responses to them. | lld:pubmed |
| pubmed-article:8458075 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8458075 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8458075 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8458075 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8458075 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8458075 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8458075 | pubmed:issn | 0143-4160 | lld:pubmed |
| pubmed-article:8458075 | pubmed:author | pubmed-author:ZuckerR SRS | lld:pubmed |
| pubmed-article:8458075 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8458075 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:8458075 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8458075 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8458075 | pubmed:pagination | 87-100 | lld:pubmed |
| pubmed-article:8458075 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:8458075 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8458075 | pubmed:articleTitle | The calcium concentration clamp: spikes and reversible pulses using the photolabile chelator DM-nitrophen. | lld:pubmed |
| pubmed-article:8458075 | pubmed:affiliation | Department of Molecular and Cell Biology, University of California, Berkeley. | lld:pubmed |
| pubmed-article:8458075 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8458075 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8458075 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:8458075 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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