| pubmed-article:8457660 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8457660 | lifeskim:mentions | umls-concept:C0005778 | lld:lifeskim |
| pubmed-article:8457660 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:8457660 | lifeskim:mentions | umls-concept:C1442792 | lld:lifeskim |
| pubmed-article:8457660 | lifeskim:mentions | umls-concept:C0369220 | lld:lifeskim |
| pubmed-article:8457660 | lifeskim:mentions | umls-concept:C0020980 | lld:lifeskim |
| pubmed-article:8457660 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:8457660 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:8457660 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:8457660 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8457660 | pubmed:dateCreated | 1993-4-27 | lld:pubmed |
| pubmed-article:8457660 | pubmed:abstractText | A novel sandwich immunoassay for measurement of soluble fibrin in plasma has been developed. For immunization we used the synthetic heptapeptide Gly-Pro-Arg-Val-Val-Glu-Arg representing the amino terminus of the alpha-chain of human fibrin. A monoclonal IgG1 antibody was obtained by conventional hybridoma technology. To increase convenience, the sandwich immunoassay was developed for the Enzymun-Test systems which are based on streptavidin pre-coated tubes. The new assay was designed with the same fibrin specific antibody both in biotinylated and in peroxidase-labelled form. Fibrin in native plasma samples could only be detected after pre-incubation of the plasma with chaotropic ions. Test results were calculated using a standard curve comprising six fibrin standards (0-50 micrograms/ml). Precision of the method was satisfactory; intra-assay CVs using plasma samples ranged between 5.0% (23.7 micrograms/ml) and 12.4% (0.2 microgram/ml). CVs of interassay precision measurements using standards as samples range between 7.3% (25.0 micrograms/ml) and 11.4% (1.0 micrograms/ml). The lower detection limit was 0.12 microgram/ml. Investigations of normal range in 70 age-matched healthy individuals resulted in a mean of 1.12 micrograms/ml. Linearity was excellent; recovery of high fibrin plasma after dilution with normal plasma was always between 100 and 107%. Fibrin specificity was due to the monoclonal antibody 2B5 used and no cross reactivity with fibrinogen, fibrinogen split products or fibrin D-dimer was observed. Fibrin fragment E1 (studied by Dempfle CE, et al. Blood Coag Fibrinol 1993; 4: 79-86) and fragments X and Y showed moderate cross reactivity in the assay and caused some overestimation of fibrin at high fibrin split product concentrations.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:8457660 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8457660 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8457660 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8457660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8457660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8457660 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8457660 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8457660 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8457660 | pubmed:issn | 0957-5235 | lld:pubmed |
| pubmed-article:8457660 | pubmed:author | pubmed-author:SchrammWW | lld:pubmed |
| pubmed-article:8457660 | pubmed:author | pubmed-author:SchullerDD | lld:pubmed |
| pubmed-article:8457660 | pubmed:author | pubmed-author:SpannaglMM | lld:pubmed |
| pubmed-article:8457660 | pubmed:author | pubmed-author:LillHH | lld:pubmed |
| pubmed-article:8457660 | pubmed:author | pubmed-author:TraunerAA | lld:pubmed |
| pubmed-article:8457660 | pubmed:author | pubmed-author:NaserWW | lld:pubmed |
| pubmed-article:8457660 | pubmed:author | pubmed-author:DessauerAA | lld:pubmed |
| pubmed-article:8457660 | pubmed:author | pubmed-author:DraegerBB | lld:pubmed |
| pubmed-article:8457660 | pubmed:author | pubmed-author:Ofenloch-Haeh... | lld:pubmed |
| pubmed-article:8457660 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8457660 | pubmed:volume | 4 | lld:pubmed |
| pubmed-article:8457660 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8457660 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8457660 | pubmed:pagination | 97-102 | lld:pubmed |
| pubmed-article:8457660 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8457660 | pubmed:meshHeading | pubmed-meshheading:8457660-... | lld:pubmed |
| pubmed-article:8457660 | pubmed:meshHeading | pubmed-meshheading:8457660-... | lld:pubmed |
| pubmed-article:8457660 | pubmed:meshHeading | pubmed-meshheading:8457660-... | lld:pubmed |
| pubmed-article:8457660 | pubmed:meshHeading | pubmed-meshheading:8457660-... | lld:pubmed |
| pubmed-article:8457660 | pubmed:meshHeading | pubmed-meshheading:8457660-... | lld:pubmed |
| pubmed-article:8457660 | pubmed:meshHeading | pubmed-meshheading:8457660-... | lld:pubmed |
| pubmed-article:8457660 | pubmed:meshHeading | pubmed-meshheading:8457660-... | lld:pubmed |
| pubmed-article:8457660 | pubmed:meshHeading | pubmed-meshheading:8457660-... | lld:pubmed |
| pubmed-article:8457660 | pubmed:meshHeading | pubmed-meshheading:8457660-... | lld:pubmed |
| pubmed-article:8457660 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8457660 | pubmed:articleTitle | A new immunoassay for soluble fibrin enables a more sensitive detection of the activation state of blood coagulation in vivo. | lld:pubmed |
| pubmed-article:8457660 | pubmed:affiliation | Boehringer Mannheim GmbH, Biochemical Research Center Tutzing, Germany. | lld:pubmed |
| pubmed-article:8457660 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8457660 | pubmed:publicationType | In Vitro | lld:pubmed |
