| pubmed-article:8455635 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8455635 | lifeskim:mentions | umls-concept:C0043393 | lld:lifeskim |
| pubmed-article:8455635 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8455635 | lifeskim:mentions | umls-concept:C0034987 | lld:lifeskim |
| pubmed-article:8455635 | lifeskim:mentions | umls-concept:C0162493 | lld:lifeskim |
| pubmed-article:8455635 | lifeskim:mentions | umls-concept:C1414404 | lld:lifeskim |
| pubmed-article:8455635 | lifeskim:mentions | umls-concept:C0439855 | lld:lifeskim |
| pubmed-article:8455635 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8455635 | pubmed:dateCreated | 1993-4-20 | lld:pubmed |
| pubmed-article:8455635 | pubmed:abstractText | The GCR1 gene product is required for maximal transcription of many yeast genes including genes encoding glycolytic enzymes. Transcription of the yeast enolase gene ENO2 is reduced 50-fold in strains carrying a gcr1 null mutation. cis-acting sequences that are sufficient for GCR1-dependent regulation of ENO2 expression were identified by using an enhancerless CYC1 promoter which is not normally dependent on GCR1 for expression. A 60-bp ENO2 sequence that was sufficient to provide high-level, GCR1-dependent transcriptional activation of the CYC1 promoter was identified. This 60-bp element could be subdivided into a 30-bp sequence containing a novel RAP1-binding site and a GCR1-binding site which did not activate CYC1 transcription and a 30-bp sequence containing a novel enhancer element that conferred moderate levels of GCR1-independent transcriptional activation. The 60-bp CGCR1-dependent upstream activator sequence is located immediately downstream from previously mapped overlapping binding sites for the regulatory proteins ABFI and RAP1. Evidence is presented that the overlapping ABFI- and RAP1-binding sites function together with sequences that bind GCR1 and RAP1 to stage transcriptional activation of ENO2 expression. | lld:pubmed |
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| pubmed-article:8455635 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:8455635 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8455635 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8455635 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8455635 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:8455635 | pubmed:author | pubmed-author:HollandM JMJ | lld:pubmed |
| pubmed-article:8455635 | pubmed:author | pubmed-author:GelfmanC MCM | lld:pubmed |
| pubmed-article:8455635 | pubmed:author | pubmed-author:WillettC ECE | lld:pubmed |
| pubmed-article:8455635 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8455635 | pubmed:volume | 13 | lld:pubmed |
| pubmed-article:8455635 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8455635 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8455635 | pubmed:pagination | 2623-33 | lld:pubmed |
| pubmed-article:8455635 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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