| pubmed-article:8439147 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8439147 | lifeskim:mentions | umls-concept:C0004036 | lld:lifeskim |
| pubmed-article:8439147 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:8439147 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:8439147 | lifeskim:mentions | umls-concept:C1154734 | lld:lifeskim |
| pubmed-article:8439147 | lifeskim:mentions | umls-concept:C1314939 | lld:lifeskim |
| pubmed-article:8439147 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:8439147 | pubmed:dateCreated | 1993-3-24 | lld:pubmed |
| pubmed-article:8439147 | pubmed:abstractText | Aflatoxins are extremely potent carcinogens produced by Aspergillus flavus and Aspergillus parasiticus. Cloning of genes in the aflatoxin pathway provides a specific approach to understanding the regulation of aflatoxin biosynthesis and, subsequently, to the control of aflatoxin contamination of food and feed. This paper reports the isolation of a gene involved in aflatoxin biosynthesis by complementation of an aflatoxin-nonproducing mutant with a wild-type genomic cosmid library of A. flavus. Strain 650-33, blocked in aflatoxin biosynthesis at the afl-2 allele, was complemented by a 32-kb cosmid clone (B9), resulting in the production of aflatoxin. The onset and profile of aflatoxin accumulation was similar for the transformed strain and the wild-type strain (NRRL 3357) of the fungus, indicating that the integrated gene is under the same control as in wild-type strains. Complementation analyses with DNA fragments from B9 indicated that the gene resides within a 2.2-kb fragment. Because this gene complements the mutated afl-2 allele, it was designated afl-2. Genetic evidence obtained from a double mutant showed that afl-2 is involved in aflatoxin biosynthesis before the formation of norsolorinic acid, the first stable intermediate identified in the pathway. Further, metabolite feeding studies with the mutant, transformed, and wild-type cultures and enzymatic activity measurements in cell extracts of these cultures suggest that afl-2 regulates gene expression or the activity of other aflatoxin pathway enzymes. This is the first reported isolation of a gene for aflatoxin biosynthesis in A. flavus. | lld:pubmed |
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| pubmed-article:8439147 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8439147 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8439147 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8439147 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8439147 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8439147 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8439147 | pubmed:issn | 0099-2240 | lld:pubmed |
| pubmed-article:8439147 | pubmed:author | pubmed-author:WoloshukC PCP | lld:pubmed |
| pubmed-article:8439147 | pubmed:author | pubmed-author:BhatnagarDD | lld:pubmed |
| pubmed-article:8439147 | pubmed:author | pubmed-author:ClevelandT... | lld:pubmed |
| pubmed-article:8439147 | pubmed:author | pubmed-author:PayneG AGA | lld:pubmed |
| pubmed-article:8439147 | pubmed:author | pubmed-author:NystromG JGJ | lld:pubmed |
| pubmed-article:8439147 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8439147 | pubmed:volume | 59 | lld:pubmed |
| pubmed-article:8439147 | pubmed:geneSymbol | afl-2 | lld:pubmed |
| pubmed-article:8439147 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8439147 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8439147 | pubmed:pagination | 156-62 | lld:pubmed |
| pubmed-article:8439147 | pubmed:dateRevised | 2010-9-13 | lld:pubmed |
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| pubmed-article:8439147 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8439147 | pubmed:articleTitle | Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus. | lld:pubmed |
| pubmed-article:8439147 | pubmed:affiliation | Department of Plant Pathology, North Carolina State University, Raleigh, 27695-7616. | lld:pubmed |
| pubmed-article:8439147 | pubmed:publicationType | Journal Article | lld:pubmed |
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