| pubmed-article:8418987 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8418987 | lifeskim:mentions | umls-concept:C0034705 | lld:lifeskim |
| pubmed-article:8418987 | lifeskim:mentions | umls-concept:C0026845 | lld:lifeskim |
| pubmed-article:8418987 | lifeskim:mentions | umls-concept:C0003483 | lld:lifeskim |
| pubmed-article:8418987 | lifeskim:mentions | umls-concept:C0001271 | lld:lifeskim |
| pubmed-article:8418987 | lifeskim:mentions | umls-concept:C0916181 | lld:lifeskim |
| pubmed-article:8418987 | lifeskim:mentions | umls-concept:C0232105 | lld:lifeskim |
| pubmed-article:8418987 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8418987 | lifeskim:mentions | umls-concept:C0392747 | lld:lifeskim |
| pubmed-article:8418987 | lifeskim:mentions | umls-concept:C0443172 | lld:lifeskim |
| pubmed-article:8418987 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8418987 | pubmed:dateCreated | 1993-2-11 | lld:pubmed |
| pubmed-article:8418987 | pubmed:abstractText | The expression of the sarcoplasmic reticulum (SR) Ca(2+)-ATPase gene and the SR Ca2+ pump function were investigated in thoracic aortas of 5- and 17-week-old normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHRs). The relative level of the two isoforms of SR Ca(2+)-ATPase mRNA expressed in the aorta (i.e., SERCA 2a and SERCA 2b) was determined by quantitative S1 nuclease protection analysis and normalized to the level of alpha-smooth muscle (alpha-Sm) actin mRNA. The level of alpha-Sm actin mRNA itself was normalized to the level of 18S ribosomal RNA using slot-blot hybridization assays. Total SR Ca2+ pump activity was estimated by measuring the rate of oxalate-supported Ca2+ uptake in homogenates. At 5 weeks, the amount of SERCA 2a and SERCA 2b mRNA, normalized to 18S ribosomal RNA, and the ratio of alpha-Sm actin mRNA to 18S RNA were identical in SHR and WKY rats. The Ca2+ pump activity was similar in the two strains of rats at 5 weeks. From 5 to 17 weeks, the amount of SERCA 2a mRNA increased in both strains while the level of SERCA 2b mRNA remained constant. The Ca2+ pump activity was unchanged in SHRs and tended to decrease in WKY rats. Accordingly, the change in the ratio of the SR Ca(2+)-ATPase mRNA isoforms does not appear to influence SR function. The level of alpha-Sm actin mRNA and SERCA 2a mRNA increased in parallel from 5 to 17 weeks in both strains.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:8418987 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8418987 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8418987 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8418987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8418987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8418987 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8418987 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8418987 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:8418987 | pubmed:issn | 0009-7330 | lld:pubmed |
| pubmed-article:8418987 | pubmed:author | pubmed-author:LevitskyD ODO | lld:pubmed |
| pubmed-article:8418987 | pubmed:author | pubmed-author:LambertFF | lld:pubmed |
| pubmed-article:8418987 | pubmed:author | pubmed-author:AngelKK | lld:pubmed |
| pubmed-article:8418987 | pubmed:author | pubmed-author:GabbianiGG | lld:pubmed |
| pubmed-article:8418987 | pubmed:author | pubmed-author:LompréA MAM | lld:pubmed |
| pubmed-article:8418987 | pubmed:author | pubmed-author:Le JemtelT... | lld:pubmed |
| pubmed-article:8418987 | pubmed:author | pubmed-author:ClergueMM | lld:pubmed |
| pubmed-article:8418987 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8418987 | pubmed:volume | 72 | lld:pubmed |
| pubmed-article:8418987 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8418987 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8418987 | pubmed:pagination | 341-8 | lld:pubmed |
| pubmed-article:8418987 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:8418987 | pubmed:meshHeading | pubmed-meshheading:8418987-... | lld:pubmed |
| pubmed-article:8418987 | pubmed:meshHeading | pubmed-meshheading:8418987-... | lld:pubmed |
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| pubmed-article:8418987 | pubmed:meshHeading | pubmed-meshheading:8418987-... | lld:pubmed |
| pubmed-article:8418987 | pubmed:meshHeading | pubmed-meshheading:8418987-... | lld:pubmed |
| pubmed-article:8418987 | pubmed:meshHeading | pubmed-meshheading:8418987-... | lld:pubmed |
| pubmed-article:8418987 | pubmed:meshHeading | pubmed-meshheading:8418987-... | lld:pubmed |
| pubmed-article:8418987 | pubmed:meshHeading | pubmed-meshheading:8418987-... | lld:pubmed |
| pubmed-article:8418987 | pubmed:meshHeading | pubmed-meshheading:8418987-... | lld:pubmed |
| pubmed-article:8418987 | pubmed:meshHeading | pubmed-meshheading:8418987-... | lld:pubmed |
| pubmed-article:8418987 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8418987 | pubmed:articleTitle | Age-related changes in sarcoplasmic reticulum Ca(2+)-ATPase and alpha-smooth muscle actin gene expression in aortas of normotensive and spontaneously hypertensive rats. | lld:pubmed |
| pubmed-article:8418987 | pubmed:affiliation | INSERM U275-LOA, ENSTA-Ecole Polytechnique, Palaiseau, France. | lld:pubmed |
| pubmed-article:8418987 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8418987 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:8418987 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:8418987 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:8418987 | lld:pubmed |
