| pubmed-article:8407049 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C0012155 | lld:lifeskim |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C0037993 | lld:lifeskim |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C1383501 | lld:lifeskim |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C0221099 | lld:lifeskim |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C0204695 | lld:lifeskim |
| pubmed-article:8407049 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:8407049 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:8407049 | pubmed:dateCreated | 1993-11-23 | lld:pubmed |
| pubmed-article:8407049 | pubmed:abstractText | Calcium-dependent signal transduction pathways of T-cell proliferation have been extensively studied in the past years. However, little is known about effects of ethanol on the calcium-dependent signal transduction pathway in T-cell proliferation. Thus, a murine model was used to determine effects of ethanol in vivo on T-cell proliferation and the intracellular free calcium concentration [Ca2+]i in response to Concanavalin A (Con A) and recombinant IL2 (rIL2) in T-cells. Splenic cells from young C57BL/6 mice, that had been fed on 3 different diets (ethanol-, maltose substitute- and standard liquid-diet) for 7-8 weeks were tested for their proliferative responses to Con A and rIL2. Concurrently, measurement was also made of [Ca2+]i in the nylon-wool-enriched resting T-cells induced by Con A and in Con-A-activated blast T-cells induced by rIL2. Our results showed that [Ca2+]i increases were seen in the splenic T-cells from three different groups of mice following Con A, but not rIL2 stimulation. However, this increase was much smaller in the splenic T-cells from ethanol-fed mice as compared to mice on maltose- or standard-diet. Furthermore, we also demonstrated that the impaired [Ca2+]i increase was seen in the T-cells of the same ethanol-fed mice having decreased the proliferative response to Con A. This reduced proliferation did not result from the presence of excessive suppressor T-cell activity. Finally, we also demonstrated that both the number of IL2 binding sites/cell and the Kd values of the low- and high-affinity IL2R on the T-cells from ethanol-fed mice were unaltered. Because evidence indicates that (1) a normal level of [Ca2+]i increase is a prerequisite for the production of IL2 by mitogen-stimulated T-cells, and (2) T-cells from ethanol-fed mice have normal capacities to produce IL2 that is the crucial growth factor controlling T-cells to progress through the cell cycle, these lines of evidence taken together with the results of this study suggest that the impairment in [Ca2+]i increases in T-cells from ethanol-fed mice may not be the primary factor contributing to the diminished T-cell proliferation in the same mice. | lld:pubmed |
| pubmed-article:8407049 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8407049 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8407049 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8407049 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8407049 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8407049 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8407049 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8407049 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8407049 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8407049 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8407049 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:8407049 | pubmed:issn | 0192-0561 | lld:pubmed |
| pubmed-article:8407049 | pubmed:author | pubmed-author:YeeHH | lld:pubmed |
| pubmed-article:8407049 | pubmed:author | pubmed-author:ChangM PMP | lld:pubmed |
| pubmed-article:8407049 | pubmed:author | pubmed-author:YamaguchiD... | lld:pubmed |
| pubmed-article:8407049 | pubmed:author | pubmed-author:NormanD CDC | lld:pubmed |
| pubmed-article:8407049 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8407049 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:8407049 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8407049 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8407049 | pubmed:pagination | 647-56 | lld:pubmed |
| pubmed-article:8407049 | pubmed:dateRevised | 2005-11-17 | lld:pubmed |
| pubmed-article:8407049 | pubmed:meshHeading | pubmed-meshheading:8407049-... | lld:pubmed |
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| pubmed-article:8407049 | pubmed:meshHeading | pubmed-meshheading:8407049-... | lld:pubmed |
| pubmed-article:8407049 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8407049 | pubmed:articleTitle | Impaired cytosolic free calcium response in splenic T-cells from mice fed with ethanol-containing diet. | lld:pubmed |
| pubmed-article:8407049 | pubmed:affiliation | Geriatric Research, Education and Clinical Center (GRECO), VA Medical Center, West Los Angeles. | lld:pubmed |
| pubmed-article:8407049 | pubmed:publicationType | Journal Article | lld:pubmed |
